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Titolo:
Up-regulated expression of HGF in rat liver cells after experimental endotoxemia: A potential pathway for enhancement of liver regeneration
Autore:
Masson, S; Daveau, M; Francois, A; Bodenant, C; Hiron, M; Teniere, P; Salier, JP; Scotte, M;
Indirizzi:
INSERM U519, Rouen, France INSERM U519 Rouen FranceINSERM U519, Rouen, France Inst Federat Rech Multidisciplinaires Peptides, Rouen, France Inst FederatRech Multidisciplinaires Peptides Rouen France uen, France Ctr Hosp & Univ, Dept Pathol, Rouen, France Ctr Hosp & Univ Rouen France tr Hosp & Univ, Dept Pathol, Rouen, France Ctr Hosp & Univ, Serv Chirurg Gen & Digest, Rouen, France Ctr Hosp & UnivRouen France , Serv Chirurg Gen & Digest, Rouen, France
Titolo Testata:
GROWTH FACTORS
fascicolo: 4, volume: 18, anno: 2001,
pagine: 237 -
SICI:
0897-7194(2001)18:4<237:UEOHIR>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEPATOCYTE-GROWTH-FACTOR; FACTOR SCATTER FACTOR; FACTOR MESSENGER-RNA; PARTIAL-HEPATECTOMY; C-MET; HEPATIC REGENERATION; ENDOTHELIAL-CELLS; KUPFFER CELLS; RECEPTOR; INTERLEUKIN-6;
Keywords:
HGF; c-Met; partial hepatectomy; liver regeneration; LPS; CD14; in situ RT-PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Daveau, M INSERM U519, Rouen, France INSERM U519 Rouen FranceINSERM U519, Rouen, France
Citazione:
S. Masson et al., "Up-regulated expression of HGF in rat liver cells after experimental endotoxemia: A potential pathway for enhancement of liver regeneration", GROW FACTOR, 18(4), 2001, pp. 237

Abstract

A lipopolysaccharide (LPS)-induced inflammation prior to an hepatic resection has been shown to enhance liver regeneration in rat. The aim of the present study was to investigate the expression of hepatocyte growth factor (HGF) and its c-Met receptor under such experimental conditions. Animals weresubmitted to a two-third hepatectomy or a LPS challenge carried out 12 h prior to resection. Non parenchymal and parenchymal cells were isolated fromlivers obtained at various times post-hepatectomy. Quantitative RT-PCR forHGF and c-Met mRNAs were performed from total liver or purified cell fractions and HGF mRNA was also analyzed by in situ RT-PCR on liver sections. A LPS challenge alone induced a marked up-regulation of HGF mRNA level in whole liver and isolated hepatocytes. Furthermore, when partial hepatectomy (PH) was preceded by a LPS challenge, an increase of HGF mRNA level was seen in whole liver and contrasted with a decreased level in non parenchymal cells. These results were confirmed by in situ RT-PCR. In isolated hepatocytesfrom endotoxemic rats, the mRNA level for the LPS-specific membranous receptor mCD14 was markedly up-regulated and even more so when LPS was followedby PH. Moreover, a TNF alpha challenge alone induced an up-regulation of HGF mRNA in hepatocytes and a down-regulation in non parenchymal cells (NPCs). Overall, when a LPS challenge is given prior to PH the major source of hepatic HGF appears to be the hepatocyte itself rather than NPCs. An autocrine HGF/c-Met loop which promotes the proliferative potential of the hepaticparenchymal cell and participates in liver regeneration is postulated.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 00:46:39