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Titolo:
Ca2+ shuttling in vesicles during tip growth in Neurospora crassa
Autore:
Torralba, S; Heath, IB; Ottensmeyer, FP;
Indirizzi:
York Univ, Dept Biol, Toronto, ON M3J 1P3, Canada York Univ Toronto ON Canada M3J 1P3 ept Biol, Toronto, ON M3J 1P3, Canada Univ Toronto, Ontario Canc Inst, Toronto, ON, Canada Univ Toronto TorontoON Canada o, Ontario Canc Inst, Toronto, ON, Canada Univ Toronto, Dept Med Biophys, Toronto, ON, Canada Univ Toronto Toronto ON Canada to, Dept Med Biophys, Toronto, ON, Canada
Titolo Testata:
FUNGAL GENETICS AND BIOLOGY
fascicolo: 3, volume: 33, anno: 2001,
pagine: 181 - 193
SICI:
1087-1845(200108)33:3<181:CSIVDT>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
OOMYCETE SAPROLEGNIA-FERAX; ENERGY-LOSS SPECTROSCOPY; GROWING HYPHAL TIPS; POLLEN-TUBE GROWTH; PLASMA-MEMBRANE; SARCOPLASMIC-RETICULUM; SYNAPTIC VESICLES; CYTOSOLIC CA2+; INOSITOL TRISPHOSPHATE; ASPERGILLUS-NIDULANS;
Keywords:
tip growth; Ca2+ gradient; Ca2+ recycling; wall vesicles; N. crassa; cyclopiazonic acid; concanamycin A;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
77
Recensione:
Indirizzi per estratti:
Indirizzo: Torralba, S York Univ, Dept Biol, 4700 Keele St, Toronto, ON M3J 1P3, Canada York Univ 4700 Keele St Toronto ON Canada M3J 1P3 1P3, Canada
Citazione:
S. Torralba et al., "Ca2+ shuttling in vesicles during tip growth in Neurospora crassa", FUNGAL G B, 33(3), 2001, pp. 181-193

Abstract

Tip-growing organisms maintain an apparently essential tip-high gradient of cytoplasmic Ca2+. In the oomycete Saprolegnia ferax, in pollen tubes and root hairs, the gradient is produced by a tip-localized Ca2+ influx from the external medium. Such a gradient is normally dispensable for Neurospora crassa hyphae, which may maintain their Ca2+ gradient by some form of internal recycling. We localized Ca2+ in N. crassa hyphae at the ultrastructural level using two techniques (a) electron spectroscopic imaging of freeze-dried hyphae and (b) pyroantimoniate precipitation. The results of both methods support the presence of Ca2+ in the wall vesicles and Golgi body equivalents, providing a plausible mechanism for the generation and maintenance of the gradient by Ca2+ shuttling in vesicles to the apex, without exogenous Ca2+ influx. Ca2+ sequestration into the vesicles seems to be dependent on Ca2+-ATPases since cyclopiazonic acid, a specific inhibitor of Ca2+ pumps, eliminated all Ca2+ deposits from the vesicles of N. crassa. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 19:34:41