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Titolo:
Changes in xylosyltransferase activity and in proteoglycan deposition in bleomycin-induced lung injury in rat
Autore:
Koslowski, R; Pfeil, U; Fehrenbach, H; Kasper, M; Skutelsky, E; Wenzel, KW;
Indirizzi:
Dresden Univ Technol, Med Fac Carl Gustav Carus, Inst Physiol Chem, D-01307 Dresden, Germany Dresden Univ Technol Dresden Germany D-01307 m, D-01307 Dresden, Germany Dresden Univ Technol, Med Fac Carl Gustav Carus, Inst Pathol, D-01307 Dresden, Germany Dresden Univ Technol Dresden Germany D-01307 l, D-01307 Dresden, Germany Dresden Univ Technol, Med Fac Carl Gustav Carus, Inst Anat, D-01307 Dresden, Germany Dresden Univ Technol Dresden Germany D-01307 t, D-01307 Dresden, Germany Tel Aviv Univ, Sackler Sch Med, Dept Pathol, IL-69978 Tel Aviv, Israel TelAviv Univ Tel Aviv Israel IL-69978 Pathol, IL-69978 Tel Aviv, Israel
Titolo Testata:
EUROPEAN RESPIRATORY JOURNAL
fascicolo: 2, volume: 18, anno: 2001,
pagine: 347 - 356
SICI:
0903-1936(200108)18:2<347:CIXAAI>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDUCED PULMONARY FIBROSIS; GROWTH-FACTOR-BETA; BRONCHOALVEOLAR LAVAGE FLUID; COLLAGEN GENE-EXPRESSION; MUSCLE ACTIN EXPRESSION; IN-SITU HYBRIDIZATION; INDUCED ALVEOLITIS; HYALURONIC-ACID; DECORIN; PROTEIN;
Keywords:
lung fibrosis; myofibroblast; proteoglycans; xylosyltransferase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Koslowski, R Dresden Univ Technol, Med Fac Carl Gustav Carus, Inst PhysiolChem, Fiedlerstr 42, D-01307 Dresden, Germany Dresden Univ Technol Fiedlerstr 42 Dresden Germany D-01307 y
Citazione:
R. Koslowski et al., "Changes in xylosyltransferase activity and in proteoglycan deposition in bleomycin-induced lung injury in rat", EUR RESP J, 18(2), 2001, pp. 347-356

Abstract

Several lines of evidence support the hypothesis of the involvement of altered proteoglycan deposition in the development of lung diseases. UDP-D-Xylose: core protein beta -D-xylosyltransferase (UDP-xylosyltransferase; EC 2.4.2.26) is a key enzyme for the glycosylation of proteoglycan core proteins. This study examined the catalytic activity of UDP-xylosyltransferase in lung tissue and in isolated fibroblasts, as well as the deposition of the proteoglycans versican, biglycan and decorin in rat lung tissue during bleomycin-induced lung injury. Rats were given, endotracheally, a single dose of bleomycin. Deposition ofproteoglycans in lung tissue was assessed by immunohistochemistry and the catalytic activity of xylosyltransferase was determined with an acceptor peptide of the sequence Q-E-E-E-G-S-G-G-G-Q-G-G as a substrate. The results show coincidence of increasing xylosyltransferase activities in lung tissue with accumulation of versican at alveolar entrance rings and in fibrotic regions in close proximity to alpha -smooth muscle actin-positive cells. In contrast, no changes in biglycan and decorin deposition in fibrotic lungs were observed, except for decorin in alveolar type II pneumocytes and alveolar macrophages. Bleomycin treatment of isolated rat lung fibroblasts resulted in a concentration-dependent increase of xylosyltransferaseactivity up to 2 mU bleomycin(.)mL(-1). The data suggest a participation of myofibroblasts with increased xylosyltransferase activities in accumulation of versican in fibrotic foci of injured lung tissue at the early stages of development of lung fibrosis.

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Documento generato il 15/08/20 alle ore 19:49:40