Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Capillaries modified by noncovalent anionic polymer adsorption for capillary zone electrophoresis, micellar electrokinetic capillary chromatography and capillary electrophoresis mass spectrometry
Autore:
Bendahl, L; Hansen, SH; Gammelgaard, B;
Indirizzi:
Royal Danish Sch Pharm, Dept Analyt & Pharmaceut Chem, DK-2100 Copenhagen,Denmark Royal Danish Sch Pharm Copenhagen Denmark DK-2100 100 Copenhagen,Denmark
Titolo Testata:
ELECTROPHORESIS
fascicolo: 12, volume: 22, anno: 2001,
pagine: 2565 - 2573
SICI:
0173-0835(200108)22:12<2565:CMBNAP>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEPARATIONS; LAYERS;
Keywords:
noncovalent adsorption of ionic polymers dynamical coating; capillary zone electrophoresis; micellar electrokinetic capillary chromatography capillary electrophoresismass spectrometry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Bendahl, L Royal Danish Sch Pharm, Dept Analyt & Pharmaceut Chem, Univ Pk 2, DK-2100 Copenhagen, Denmark Royal Danish Sch Pharm Univ Pk 2 CopenhagenDenmark DK-2100 rk
Citazione:
L. Bendahl et al., "Capillaries modified by noncovalent anionic polymer adsorption for capillary zone electrophoresis, micellar electrokinetic capillary chromatography and capillary electrophoresis mass spectrometry", ELECTROPHOR, 22(12), 2001, pp. 2565-2573

Abstract

A simple coating procedure for generation of a high and pH-independent electroosmotic flow in capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chromatography (MEKC) is described. The bilayer coatingwas formed by noncovalent adsorption of the ionic polymers Polybrene and poly(vinylsulfonate) (PVS). A stable dynamic coating was formed when PVS wasadded to the background electrolyte. Thus, when the PVS concentration in the background electrolyte was optimized for CZE (0.01 %), the EOF differed less than 0.3% after 54 runs. The electroosmotic mobility in the coated capillaries was (4.9 +/- 0.1) X 10(-4) cm(2)V(-1)s(-1) in a pH-range of 2-10 (ionic strength = 30 mM). When alkaline compounds were used as test substances intracapillary and intercapillary migration time variations (n = 6) wereless than 1% relative standard deviation (RSD) and 2% RSD, respectively inthe entire pH range. The coating was fairly stable in the presence of sodium dodecyl sulfate, and this made it possible to perform fast MEKC separations at low pH. When neutral compounds were used as test substances, the intracapillary migration time variations (n = 6) were less than 2% RSD in a pHrange of 2-9. In addition to fast CZE and MEKC separations at low pH, analysis of the alkaline compounds by CE-MS was also possible.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 20:27:46