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Titolo:
Characterization of the rat Gad67 gene promoter reveals elements importantfor basal transcription and glucose responsiveness
Autore:
Pedersen, AA; Videbaek, N; Skak, K; Petersen, HV; Michelsen, BK;
Indirizzi:
Hagedorn Res Inst, DK-2820 Gentofte, Denmark Hagedorn Res Inst Gentofte Denmark DK-2820 st, DK-2820 Gentofte, Denmark
Titolo Testata:
DNA SEQUENCE
fascicolo: 6, volume: 11, anno: 2001,
pagine: 485 - 499
SICI:
1042-5179(2001)11:6<485:COTRGG>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUTAMIC-ACID DECARBOXYLASE; ETS ONCOGENE FAMILY; PANCREATIC-ISLETS; MESSENGER-RNAS; INSULIN GENE; NOD MICE; EXPRESSION; PEA3; ACTIVATOR; ENHANCER;
Keywords:
islets of Langerhans; PEA3; Sp1; Egr-1; Pdx-1; E-box;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Michelsen, BK Hagedorn Res Inst, Niels Steensensvej 6, DK-2820 Gentofte, Denmark Hagedorn Res Inst Niels Steensensvej 6 Gentofte Denmark DK-2820
Citazione:
A.A. Pedersen et al., "Characterization of the rat Gad67 gene promoter reveals elements importantfor basal transcription and glucose responsiveness", DNA SEQ, 11(6), 2001, pp. 485-499

Abstract

GAD65 and GAD67 are two isoforms of the enzyme glutamic acid decarboxylasewhich catalyze the production of GABA from glutamate, primarily in the brain. However, GAD and GABA also prevail in the retina, testes and islets of Langerhans. The main function of GABA is in neurotransmission, and it is involved in paracrine signalling in islets, but has also been suggested to play a role as a trophic factor in synaptogenesis and to be an important metabolite feeding into the tricarboxylic acid cycle via the GABA-shunt. Both GAD isoforms are subject to regulation, e.g. by synaptic activity. GAD65 is regulated at the level of enzyme activity by association and dissociation from its cofactor, PLP, whereas GAD67 is controlled at the level of its mRNA. To study this process in further detail, we have isolated and characterized the 5 ' -flanking region of the rat GAD67 gene. We report the transcriptional initiation sites and promoter sequences important for expression in islet beta -cells and C6 glioma cells, and demonstrate that the GAD67 promoter harbors elements that are responsive to glucose in primary islet cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 19:23:06