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Titolo:
N-phenacylthiazolium bromide decreases renal and increases urinary advanced glycation end products excretion without ameliorating diabetic nephropathy in C57BL/6 mice
Autore:
Schwedler, SB; Verbeke, P; Bakala, H; Weiss, MF; Vilar, J; Depreux, P; Fourmaintraux, E; Striker, LJ; Striker, GE;
Indirizzi:
Univ Miami, Dept Med, Div Nephrol, Lab Renal Cell Biol, Miami, FL USA UnivMiami Miami FL USA Div Nephrol, Lab Renal Cell Biol, Miami, FL USA Univ Paris, Lab Biol & Cellular Biochem Ageing, F-75252 Paris, France UnivParis Paris France F-75252 ar Biochem Ageing, F-75252 Paris, France Case Western Reserve Univ, Sch Med, Dept Med, Cleveland, OH 44106 USA CaseWestern Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA Univ Paris, INSERM, U319, F-75252 Paris, France Univ Paris Paris France F-75252 ris, INSERM, U319, F-75252 Paris, France Univ Lille, Inst Pharmaceut Chem, Lille, France Univ Lille Lille FranceUniv Lille, Inst Pharmaceut Chem, Lille, France
Titolo Testata:
DIABETES OBESITY & METABOLISM
fascicolo: 4, volume: 3, anno: 2001,
pagine: 230 - 239
SICI:
1462-8902(200108)3:4<230:NBDRAI>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN CROSS-LINKS; MAILLARD REACTION; IMMUNOHISTOCHEMICAL DETECTION; IMMUNOCHEMICAL DETECTION; SKIN COLLAGEN; N-EPSILON-(CARBOXYMETHYL)LYSINE; AMINOGUANIDINE; ACCUMULATION; PATHOGENESIS; ENDPRODUCTS;
Keywords:
N-phenacylthiazolium bromide (PTB); advanced glycation end products (AGE); diabetic nephropathy; C57BL/6 mice;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Schwedler, SB Univ Wurzburg, Med Klin, Nephrol Abt, Bau 4,Josef Schneider Str 2, D-97080Wurzburg, Germany Univ Wurzburg Bau 4,Josef Schneider Str 2 Wurzburg Germany D-97080
Citazione:
S.B. Schwedler et al., "N-phenacylthiazolium bromide decreases renal and increases urinary advanced glycation end products excretion without ameliorating diabetic nephropathy in C57BL/6 mice", DIABET OB M, 3(4), 2001, pp. 230-239

Abstract

Aims: Advanced glycation end products (AGE), which form from the non-enzymatic reaction of proteins and sugars, have been implicated in the pathogenesis of diabetic nephropathy. Recently, a compound [N-phenacylthiazolium bromide (PTB)] has been described which cleaves alpha,beta -dicarbonyl compounds. In the present study we used diabetic C57BL/6 mice to determine if PTB altered renal AGE levels and reduced diabetic glomerulosclerosis. Methods: Mice with stable hyperglycaemia induced by streptozotocin were given daily subcutaneous injections of either PTB (10 mug/g) or saline for 12weeks. Renal-collagen bound AGE and urinary AGE-peptides were measured by ELISA using an anti-AGE-RNase antibody. Renal collagen-released N-epsilon(carboxymethyl)lysine (CML) and pentosidine were determined by high pressure liquid chromatography (HPLC). Glomerular lesions (volume and mesangial/total surface area) were evaluated by computer-assisted image analysis. We determined urinary protein/ creatinine ratio as a functional parameter. AGE localization was examined by immunohistochemistry using the anti-AGE-RNase antibody. Results: Renal collagen-bound AGE were decreased and urinary AGE excretionwas increased in PTB-treated diabetic mice. However, collagen-released CMLand pentosidine were similar in both groups. Glomerular histology and morphometric analysis revealed also no differences between PTB-and saline-treated diabetic mice. The urinary protein/creatinine ratio was unaffected by PTB-treatment. AGE staining by anti-AGE-RNase antibody was present in Bowman's capsules, glomerular basement membranes and cortical tubules. It was decreased in all structures in PTB-treated diabetic mice. Conclusion: In summary, PTB decreased renal AGE accumulation but did not ameliorate glomerular lesions or proteinuria. Thus, cleavage of AGE by PTB is not sufficient to prevent development of diabetic nephropathy in C57BL/6 mice.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/10/20 alle ore 14:11:00