Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
TGF beta 2 activation status during cardiac morphogenesis
Autore:
McCormick, KM;
Indirizzi:
SUNY Buffalo, Dept Phys Therapy Exercise & Nutr Sci, Buffalo, NY 14214 USASUNY Buffalo Buffalo NY USA 14214 rcise & Nutr Sci, Buffalo, NY 14214 USA
Titolo Testata:
DEVELOPMENTAL DYNAMICS
fascicolo: 1, volume: 222, anno: 2001,
pagine: 17 - 25
SICI:
1058-8388(200109)222:1<17:TB2ASD>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-BETA; CHICKEN-EMBRYO; DEVELOPMENTAL EXPRESSION; 6-PHOSPHATE RECEPTORS; CELL-TRANSFORMATION; MOUSE EMBRYOGENESIS; HEART; GENE; RNA; THROMBOSPONDIN-1;
Keywords:
latent TGF beta; chick embryo; myocardium; heart development; morphogenesis; cardiogenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: McCormick, KM SUNY Buffalo, Dept Phys Therapy Exercise & Nutr Sci, 405 Kimball Tower,3435 Main St, Buffalo, NY 14214 USA SUNY Buffalo 405 Kimball Tower,3435 Main St Buffalo NY USA 14214
Citazione:
K.M. McCormick, "TGF beta 2 activation status during cardiac morphogenesis", DEV DYNAM, 222(1), 2001, pp. 17-25

Abstract

Transforming growth factor beta (TGF beta) is secreted as a biologically inactive complex by many cell types in vitro, but little is known of TGF beta 's activation status in vivo. This study examined the in vivo expression of active and total (active + acid-activatable) TGF beta2 in embryonic chicken hearts during cardiac morphogenesis (Hamburger-Hamilton stage 10-24). The concentration of TGF beta2 was measured by an enzyme-linked immunoassay that recognized active TGF beta2. Whole heart homogenates, were either leftuntreated to measure active TGF beta2 or treated with acid before assay tomeasure total (active + acid-activatable) TGF beta2. Total TGF beta2 concentration increased more than 16-fold between stage 10/11 and stage 24. Active TGF beta2 concentration was highest at stage 14/15, but overall remainedrelatively constant varying at most by 2.8-fold. When expressed relative to total TGF beta2, the amount of active TGF beta2 progressively declined from 70% in stage 10/11 hearts to 7% in stage 24 hearts. The distribution of active and total TGF beta2 was examined by immunostaining with an antibody against active TGF beta2. Before immunostaining, sections were either treated with acid or left untreated to determine the distribution of total and active TGF beta2, respectively. Active TGF beta2 immunostaining was first detected in the endothelium, myocardium, and cardiac jelly of stage 14 hearts. Acid treatment had no effect on the distribution or intensity of immunostaining at this stage. Faint, active TGF beta2 immunostaining was restrictedto the ventricular myocardium in stage 18 hearts. Acid treatment resulted in a marked increase in staining intensity in the ventricle, but no staining was observed in the atrium or outflow tract. In stage 24 hearts, faint active TGF beta2 staining was detected in the ventricle before acid treatment. After acid treatment, patches of intense punctate stain were found in allregions of the embryonic heart. Increases in TGF beta2 concentration and immunostaining intensity after acidification suggest that a significant amount of TGF beta2 is in the latent form. Stage-dependent differences in activation status suggest that activation may be a developmentally regulated process in the chick heart and support the notion that activation is an important step in regulating TGF beta actions in vivo. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 07:30:10