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Titolo:
Evidence for a novel thrombopoietin signalling event: Activation of protein kinase a in human megakaryoblastic CMK cells
Autore:
Sauer, M; Tausch, S; Zieger, M; Zintl, F; Nowak, G; Kaufmann, R;
Indirizzi:
Univ Jena, Fac Med, Res Grp Pharmacol Haemostaseol, D-07747 Jena, Germany Univ Jena Jena Germany D-07747 macol Haemostaseol, D-07747 Jena, Germany Univ Jena, Fac Med, Div Pediat Oncol & Hematol, Jena, Germany Univ Jena Jena Germany c Med, Div Pediat Oncol & Hematol, Jena, Germany Univ Minnesota, Sch Med, Div Pediat Hematol Oncol & Blood & Marrow Transpl, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 ranspl, Minneapolis, MN 55455 USA
Titolo Testata:
CYTOKINE
fascicolo: 2, volume: 15, anno: 2001,
pagine: 75 - 79
SICI:
1043-4666(20010721)15:2<75:EFANTS>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAMMALIAN ADENYLYL CYCLASES; ARTERIAL SMOOTH-MUSCLE; HUMAN BLOOD-PLATELETS; TYROSINE PHOSPHORYLATION; RECOMBINANT THROMBOPOIETIN; SIGNALING PATHWAYS; CYCLIC-AMP; MPL-LIGAND; TRANSDUCTION; CAMP;
Keywords:
cAMP; ERK 1/2; mitogen-activated protein kinases; TPO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Kaufmann, R Univ Jena, Fac Med, Res Grp Pharmacol Haemostaseol, Drackendorfer Str 1, D-07747 Jena, Germany Univ Jena Drackendorfer Str 1 Jena Germany D-07747 a, Germany
Citazione:
M. Sauer et al., "Evidence for a novel thrombopoietin signalling event: Activation of protein kinase a in human megakaryoblastic CMK cells", CYTOKINE, 15(2), 2001, pp. 75-79

Abstract

Thrombopoietin (TPO) plays a crucial role in megakaryocyte development. TPO signalling, which is mediated by its receptor Mpl, includes Janus kinase,(JAK) signal transducer and activator of transcription (STAT) and Shc/Ras/mitogen-activated protein kinase (MAPK) pathways. The precise nature of these signalling routes has not been clarified in detail up until now. We investigated the effect of TPO on activation of cAMP-dependent protein kinase (PKA) and its involvement in MAPK signalling in human megakaryoblastic leukaemia CMK cells. For estimation of PKA activity, phosphorylation of a PKA-specific peptide substrate was assayed in CMK cell lysates. Since activation of PKA is associated with translocation of its catalytic subunit alpha (C-PKA) into the cell nucleus, Western blot analysis of nuclear fractions with an anti-C-PKA antibody was additionally performed. The activation of TPO-induced MAPK activation and the effect of the PKA inhibitor H-89 was measuredusing immunoblotting with a monoclonal anti-pERK antibody. TPO enhanced cAMP and induced activation of PKA in CMK cells. In addition, H-89 partly blocked TPO-induced MAPK activation in CMK cells. Our results indicate a novelTPO-triggered signalling event, activation of the cAMP/PKA pathway in human megakaryoblastic CMK cells. This signal transduction route seems to be involved in TPO-induced MAPK signaling. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 01:31:55