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Titolo:
An endogenous adrenoceptor ligand potentiates excitatory synaptic transmission in cultured hippocampal neurons
Autore:
Aubert, M; Guiramand, J; Croce, A; Roch, G; Szafarczyk, A; Vignes, M;
Indirizzi:
Univ Montpellier 2, Lab Plast Cerebrale, UMR 5102, CNRS, F-34095 Montpellier 05, France Univ Montpellier 2 Montpellier France 05 F-34095 Montpellier 05, France
Titolo Testata:
CEREBRAL CORTEX
fascicolo: 9, volume: 11, anno: 2001,
pagine: 878 - 887
SICI:
1047-3211(200109)11:9<878:AEALPE>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADRENERGIC-RECEPTOR ACTIVATION; POLYMERASE CHAIN-REACTION; IN-SITU HYBRIDIZATION; BETA-2-ADRENERGIC RECEPTORS; NORADRENERGIC INNERVATION; INVERSE AGONISM; GENE-EXPRESSION; RAT-BRAIN; ENHANCEMENT; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Vignes, M Univ Montpellier 2, Lab Plast Cerebrale, UMR 5102, CNRS, F-34095Montpellier 05, France Univ Montpellier 2 Montpellier France 05 ontpellier 05, France
Citazione:
M. Aubert et al., "An endogenous adrenoceptor ligand potentiates excitatory synaptic transmission in cultured hippocampal neurons", CEREB CORT, 11(9), 2001, pp. 878-887

Abstract

Noradrenergic inputs modulate hippocampal function via distinct receptors. In hippocampal neuronal cultures, mRNA expression of adrenoceptor subtypesis maintained from 1 day in vitro (DIV) to 22 DIV. Noradrenaline dose-dependently stimulates phosphoinositide (PI) breakdown in both immature and mature cultures through the activation of al receptors. At 22 DIV, basal PI breakdown depends on excitatory synaptic activity since it is decreased by tetrodotoxin or glutamate receptor antagonists. At 22 DIV, a similar decreaseof basal PI breakdown is also observed with alpha1, alpha2 or beta adrenoceptor antagonists. These effects are not additive with that produced by tetrodotoxin. Adrenergic antagonists also strongly reduce spontaneous excitatory post-synaptic currents (sEPSC) as evidenced by whole call recording. Therefore, in hippocampal cultures, excitatory transmission is modulated by a tonic activation of adrenoceptors probably produced by an endogenous ligand. Indeed, (i) the depletion of catecholamine pools by reserpine also decreases both basal PI metabolism and sEPSC; (ii) hippocampal neurons possess both tyrosine hydroxylase (TH) and dopamine-beta -hydroxylase mRNAs, encodingenzymes required for catecholamine synthesis; and (iii) some hippocampal neurons show TH-immunoreactivity. TH-positive cells are also detected in E18hippocampal sections. Thus, cultured hippocampal neurons synthesize and release an adrenergic-like ligand, which tonically potentiates excitatory synaptic transmission in mature cultures.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 12:18:40