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Titolo:
Cytosolic Ca2+ levels and DNA synthesis of human umbilical vein endothelial cell cultures are unresponsive to thrombopoietin treatment
Autore:
Metzen, E; Giesen, G; Fandrey, J; Jelkmann, W;
Indirizzi:
Med Univ Lubeck, Inst Physiol, D-23538 Lubeck, Germany Med Univ Lubeck Lubeck Germany D-23538 Physiol, D-23538 Lubeck, Germany
Titolo Testata:
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY
fascicolo: 4, volume: 11, anno: 2001,
pagine: 197 - 202
SICI:
1015-8987(2001)11:4<197:CCLADS>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
LINKED-IMMUNOSORBENT-ASSAY; MESSENGER-RNA EXPRESSION; GROWTH-FACTOR; C-MPL; RECEPTOR; ERYTHROPOIETIN; CANCER; MEGAKARYOCYTOPOIESIS; INTERNALIZATION; ANGIOGENESIS;
Keywords:
thrombopoietin; endothelial cells; platelets; MPL-ligand; calcium;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Jelkmann, W Med Univ Lubeck, Inst Physiol, Ratzeburger Allee 160, D-23538 Lubeck, Germany Med Univ Lubeck Ratzeburger Allee 160 Lubeck Germany D-23538
Citazione:
E. Metzen et al., "Cytosolic Ca2+ levels and DNA synthesis of human umbilical vein endothelial cell cultures are unresponsive to thrombopoietin treatment", CELL PHYS B, 11(4), 2001, pp. 197-202

Abstract

The hormone thrombopoietin (TPO) induces proliferation of megakaryocytic progenitors and augments agonist-induced mobilization of Ca2+ in platelets. Because the action of TPO is not restricted to the megakaryocytic lineage, we studied the occurrence of TPO receptor mRNA and protein, and effects of TPO on cytosolic Ca2+ levels and DNA synthesis in human umbilical vein endothelial cell cultures (HUVECs). Polymerase chain reaction following reversetranscription (RT-PCR) of total mRNA revealed that TPO receptor (MPL) mRNAwas expressed only at low level in our samples. TPO receptor protein was not detectable in HUVEC lysates investigated by immunoprecipitation and immunoblotting. In contrast to vascular endothelial growth factor (VEGF), TPO did neither alter fura2 fluorescence as a measure of cytosolic Ca2+ levels nor increase 5-bromo-2 ' -deoxyuridine incorporation into DNA of HUVECs. In conclusion, our data demonstrate that HUVECs are neither structurally nor functionally responsive to TPO. Copyright (C) 2001 S. Karger AG, Basel.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 14:26:50