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Titolo:
Culture in low levels of oxygen enhances in vitro proliferation potential of satellite cells from old skeletal muscles
Autore:
Chakravarthy, MV; Spangenburg, EE; Booth, FW;
Indirizzi:
Univ Missouri, Dept Vet Biomed Sci, Columbia, MO 65211 USA Univ Missouri Columbia MO USA 65211 et Biomed Sci, Columbia, MO 65211 USA Univ Missouri, Dalton Cardiovasc Inst, Columbia, MO 65211 USA Univ Missouri Columbia MO USA 65211 rdiovasc Inst, Columbia, MO 65211 USA Univ Texas, Sch Med, Dept Integrat Biol, Houston, TX 77054 USA Univ TexasHouston TX USA 77054 Dept Integrat Biol, Houston, TX 77054 USA
Titolo Testata:
CELLULAR AND MOLECULAR LIFE SCIENCES
fascicolo: 8, volume: 58, anno: 2001,
pagine: 1150 - 1158
SICI:
1420-682X(200107)58:8<1150:CILLOO>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
REPLICATIVE LIFE-SPAN; GENE-THERAPY; GROWTH; EXPRESSION; DIFFERENTIATION; P27(KIP1); INVITRO; PATHWAY; HYPOXIA; HYPERTROPHY;
Keywords:
hypoxia; stem cell; cellular senescence; aging; cell cycle; Akt;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Booth, FW Univ Missouri, Dept Vet Biomed Sci, 1600 E Rollins St E,102 Vet Med Bldg, Columbia, MO 65211 USA Univ Missouri 1600 E Rollins St E,102 Vet Med Bldg Columbia MO USA 65211
Citazione:
M.V. Chakravarthy et al., "Culture in low levels of oxygen enhances in vitro proliferation potential of satellite cells from old skeletal muscles", CELL MOL L, 58(8), 2001, pp. 1150-1158

Abstract

The proliferation ability of satellite cells (considered the 'stem cells' of mature myofibers) declines with increasing age when cultured under standard cell culture conditions of 21 % oxygen. However, actual oxygen levels in the intact myofiber in vivo are an order of magnitude lower. No studies to date have addressed the issue of whether culturing satellite cells from old muscles under more 'physiologic' conditions would enhance their proliferation and/or differentiation ability. Therefore, we analyzed satellite cells derived from 31-month-old rats in standard cultures with 21 % O-2 and in lowered (similar to3 %) O-2. Under the lowered O-2 conditions, we noted a remarkable increase in the percentage of large-sized colonies, activation ofcell cycle progression factors, phosphorylation of Akt, and downregulationof the cell cycle inhibitor p27(Kip1). These data suggest that lower O-2 levels provide a milieu that stimulates proliferation by allowing continued cell cycle progression, to result ultimately in the enhanced in vitro replicative life span of the old satellite cells. Such a method therefore provides an improved means for the ex vivo generation of progenitor satellite cell populations for potential therapeutic stem cell transplantation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 19:41:21