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Titolo:
In vitro organization of biohybrid rat liver tissue incorporating growth factor- and hormone-releasing biodegradable polymer microcapsules
Autore:
Sakai, Y; Furukawa, K; Ushida, T; Tateishi, T; Suzuki, M;
Indirizzi:
Univ Tokyo, Inst Ind Sci, Meguro Ku, Tokyo 1538505, Japan Univ Tokyo Tokyo Japan 1538505 Ind Sci, Meguro Ku, Tokyo 1538505, Japan Agcy Ind Sci & Technol, Natl Inst Adv Interdisciplinary Res, 3D Cell Tissue Engn Grp, Tsukuba, Ibaraki 3058562, Japan Agcy Ind Sci & Technol TsukubaIbaraki Japan 3058562 araki 3058562, Japan Univ Tokyo, Grad Sch Engn, Tissue Engn Div, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 Engn Div, Bunkyo Ku, Tokyo 1130033, Japan
Titolo Testata:
CELL TRANSPLANTATION
fascicolo: 4-5, volume: 10, anno: 2001,
pagine: 479 - 483
SICI:
0963-6897(2001)10:4-5<479:IVOOBR>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
PORCINE HEPATOCYTE SPHEROIDS; BIOARTIFICIAL LIVER; DELIVERY;
Keywords:
biodegradable polymer microcapsule; growth factor; rat hepatocyte; biohybrid liver tissue;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Sakai, Y Univ Tokyo, Inst Ind Sci, Meguro Ku, 4-6-1 Komaba, Tokyo 1538505,Japan Univ Tokyo 4-6-1 Komaba Tokyo Japan 1538505 Tokyo 1538505, Japan
Citazione:
Y. Sakai et al., "In vitro organization of biohybrid rat liver tissue incorporating growth factor- and hormone-releasing biodegradable polymer microcapsules", CELL TRANSP, 10(4-5), 2001, pp. 479-483

Abstract

To enhance the liver-specific functions of rat hepatocyte aggregates without the addition of exogenous growth factors, polylactic acid-polyglycolic acid (PLGA)/gelatin microcapsules that release insulin, dexamethasone, epidermal growth factors, and glucagon were prepared and incorporated into the hepatocyte aggregates in suspension culture. Precoating the capsules with fibronectin enhanced the incorporation of the microcapsules into the hepatocyte aggregates. In a growth factor- and hormone-free culture medium, these microcapsule-containing aggregates showed a sustained cell number and an ammonium detoxification capacity compared with two types of control culture. One was the culture of microcapsule-free aggregates with albumin-containing control capsules and the other was the culture of capsule-free aggregates that were supplied with the same factors and hormones from the culture medium at concentration levels expected from the release kinetics of the microcapsules. Our new methodology demonstrates that the performance and duration of bioartificial liver systems can be enhanced due to a more efficient maintenance of cell number by using such growth factor- and hormone-releasing microcapsules.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 03:36:41