Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Neurotrophic factor-secreting cell grafting for cerebral ischemia: Preliminary report
Autore:
Fujiwara, K; Date, I; Shingo, T; Yoshida, H; Kobayashi, K; Takeuchi, A; Tamiya, T; Ohmoto, T;
Indirizzi:
Okayama Univ, Sch Med, Dept Neurol Surg, Okayama 7008558, Japan Okayama Univ Okayama Japan 7008558 t Neurol Surg, Okayama 7008558, Japan
Titolo Testata:
CELL TRANSPLANTATION
fascicolo: 4-5, volume: 10, anno: 2001,
pagine: 419 - 422
SICI:
0963-6897(2001)10:4-5<419:NFCGFC>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
PARKINSONS-DISEASE; ARTERY OCCLUSION; NEURAL TRANSPLANTATION; HEMIPARKINSONIAN RATS; BEHAVIORAL RECOVERY; TOPICAL APPLICATION; TROPHIC FACTORS; BRAIN INJURY; GROWTH; LINE;
Keywords:
basic fibroblast growth factor (bFGF); encapsulation; cell grafting; ischemia;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Fujiwara, K Okayama Univ, Sch Med, Dept Neurol Surg, 2-5-1 Shikata Cho, Okayama 7008558, Japan Okayama Univ 2-5-1 Shikata Cho Okayama Japan 7008558 8, Japan
Citazione:
K. Fujiwara et al., "Neurotrophic factor-secreting cell grafting for cerebral ischemia: Preliminary report", CELL TRANSP, 10(4-5), 2001, pp. 419-422

Abstract

In this experiment, we examined a possible protective effect of encapsulated neurotrophic factor-secreting cell-rafting for ischemic injury. We established a basic fibroblast growth factor (bFGF)-secreting cell line by genetic manipulation. We enveloped these cells into polymer capsules, which consist of a semipermeable membrane, and implanted them into the right striatumof rats. At 6 days after implantation, these rats received right middle cerebral artery occlusion (MCAO) using interluminal suture technique. At 24 hafter MCAO, rats were sacrificed and their cerebral infarction volume was determined by 2,3,5-triphenyltetrazolium chloride (TTC) staining and image analysis. We found approximately 30% reduction in infarct volume in the encapsulated bFGF-secreting cell grafting groups vs. the encapsulated naive BHK cell grafting group or the without implantation group. We measured bFGF secretion from encapsulated bFGF-secreting cells using enzyme-linked immunosorbent assay (ELISA). The retrieved capsules continued to secrete bFGF. There was no significant difference of bFGF secretion between the capsules before and after transplantation. A large number of viable BHK-bFGF cells was observed within the full length of the retrieved capsule. These results indicate that encapsulated bFGF-secreting cell grafting exerts a protective effect on ischemic injury.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 22:00:45