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Titolo:
Rapidly functional immobilization of immortalized human hepatocytes using cell adhesive GRGDS peptide-carrying cellulose microspheres
Autore:
Kobayashi, N; Taguchi, T; Noguchi, H; Okitsu, T; Totsugawa, T; Watanabe, T; Matsumura, T; Fujiwara, T; Urata, H; Kishimoto, N; Hayashi, N; Nakaji, S; Murakami, T; Tanaka, N;
Indirizzi:
Okayama Univ, Sch Med, Dept Surg 1, Okayama 7008558, Japan Okayama Univ Okayama Japan 7008558 , Dept Surg 1, Okayama 7008558, Japan Okayama Univ, Sch Med, Dept Anat 2, Okayama 7008558, Japan Okayama Univ Okayama Japan 7008558 , Dept Anat 2, Okayama 7008558, Japan Okayama Univ, Sch Med, Cent Res Lab, Okayama 7008558, Japan Okayama Univ Okayama Japan 7008558 Cent Res Lab, Okayama 7008558, Japan Kuraray Co Ltd, Dept Med Prod, Okayama 7108622, Japan Kuraray Co Ltd Okayama Japan 7108622 pt Med Prod, Okayama 7108622, Japan
Titolo Testata:
CELL TRANSPLANTATION
fascicolo: 4-5, volume: 10, anno: 2001,
pagine: 387 - 392
SICI:
0963-6897(2001)10:4-5<387:RFIOIH>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
HYBRID ARTIFICIAL LIVER; LINES; ANTIGEN; FAILURE; SYSTEM; TISSUE; GENE;
Keywords:
immortal hepatocytes; cellulose microspheres; artificial liver;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Kobayashi, N Okayama Univ, Sch Med, Dept Surg 1, 2-5-1 Shikata Cho, Okayama 7008558, Japan Okayama Univ 2-5-1 Shikata Cho Okayama Japan 7008558 , Japan
Citazione:
N. Kobayashi et al., "Rapidly functional immobilization of immortalized human hepatocytes using cell adhesive GRGDS peptide-carrying cellulose microspheres", CELL TRANSP, 10(4-5), 2001, pp. 387-392

Abstract

With the development of biotechnology, hepatic support by a hybrid artificial liver (HAL) using hepatocytes has been given much attention. Because the availability of human livers is limited, we have established a tightly regulated immortal human hepatocyte cell line, NKNT-3, for developing HAL. Because hi.-h-density cell culture allows the compactness of the HAL device and its easy use under emergency circumstances, we have developed cell adhesive GRGDS peptide-containing cellulose microspheres (GRGDS/CMS). The GRGDS/CMS efficiently immobilized NKNT-3 cells within 24 h in a stirred suspension culture. Electron microscopic examinations demonstrated glycogen granulesand well-developed endoplasmic reticulum and mitochondria in NKNT-3 cells attached to the GRGDS/CMS. The cells showed ammonia clearance activity, whereas HepG2-transformed human liver cells did not remove the loaded ammonia. An efficient adenoviral delivery of the lacZ reporter gene was performed in GRGDS/CMS-immobilized NKNT-3 cells. In this study we present rapid immobilization of NKNT-3 immortal human hepatocytes using cellulose microspheres carrying GRGDS peptides. These microspheres satisfied immediate preparationof NKNT-3 cells in sufficient quantity and of adequate quality.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 00:24:46