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Titolo:
Cre/loxP-based reversible immortalization of human hepatocytes
Autore:
Kobayashi, N; Noguchi, H; Westerman, KA; Watanabe, T; Matsumura, T; Totsugawa, T; Fujiwara, T; Leboulch, P; Tanaka, N;
Indirizzi:
Okayama Univ, Sch Med, Dept Surg 1, Okayama 7008558, Japan Okayama Univ Okayama Japan 7008558 , Dept Surg 1, Okayama 7008558, Japan Harvard Univ, MIT, Div Hlth Sci & Technol, Cambridge, MA 02139 USA HarvardUniv Cambridge MA USA 02139 ci & Technol, Cambridge, MA 02139 USA
Titolo Testata:
CELL TRANSPLANTATION
fascicolo: 4-5, volume: 10, anno: 2001,
pagine: 383 - 386
SICI:
0963-6897(2001)10:4-5<383:CRIOHH>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE LIVER-FAILURE; TRANSPLANTATION; SYSTEM;
Keywords:
reversible immortalization; human hepatocytes; Cre/loxP system; simian virus 40 large T antigen;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
8
Recensione:
Indirizzi per estratti:
Indirizzo: Kobayashi, N Okayama Univ, Sch Med, Dept Surg 1, 2-5-1 Shikata Cho, Okayama 7008558, Japan Okayama Univ 2-5-1 Shikata Cho Okayama Japan 7008558 , Japan
Citazione:
N. Kobayashi et al., "Cre/loxP-based reversible immortalization of human hepatocytes", CELL TRANSP, 10(4-5), 2001, pp. 383-386

Abstract

An ideal alternative to the primary human hepatocytes for hepatocyte transplantation would be to use a clonal. cell line that grows economically in culture and exhibits the characteristics of differentiated, nontransformed hepatocytes following transplantation. The purpose of the present studies was to establish a reversibly immortalized human hepatocyte cell line. Human hepatocytes were immortalized with a retroviral vector SSR#69 expressing simian virus 40 large T antigen (SV40Tag) gene flanked by a pair of loxP recombination targets. One of the resulting clones, NKNT-3, showed morphological characteristics of liver parenchymal cells and expressed the genes of differentiated liver functions. NKNT-3 cells offered unlimited availability. After an adenoviral delivery of Cre recombinase and subsequent differential selection, efficient removal of SV40Tag from NKNT-3 cells was performed. Here we represent that elimination of the retrovirally transferred SV40Tag gene can be excised by adenovirus-mediated site-specific recombination.

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Documento generato il 30/11/20 alle ore 11:57:48