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Titolo:
Successful retroviral gene transfer of simian virus 40 T antigen and herpes simplex virus-thymidine kinase into human hepatocytes
Autore:
Kobayashi, N; Noguchi, H; Westerman, KA; Watanabe, T; Matsumura, T; Totsugawa, T; Fujiwara, T; Leboulch, P; Tanaka, N;
Indirizzi:
Okayama Univ, Sch Med, Dept Surg 1, Okayama 7008558, Japan Okayama Univ Okayama Japan 7008558 , Dept Surg 1, Okayama 7008558, Japan Harvard Univ, MIT, Div Hlth Sci & Technol, Cambridge, MA 02139 USA HarvardUniv Cambridge MA USA 02139 ci & Technol, Cambridge, MA 02139 USA
Titolo Testata:
CELL TRANSPLANTATION
fascicolo: 4-5, volume: 10, anno: 2001,
pagine: 377 - 381
SICI:
0963-6897(2001)10:4-5<377:SRGTOS>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMORTALIZED HUMAN HEPATOCYTES; ACUTE LIVER-FAILURE; TRANSPLANTATION; CELL;
Keywords:
immortalized human hepatocyte; retroviral gene transfer; simian virus 40 large T antigen;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Kobayashi, N Okayama Univ, Sch Med, Dept Surg 1, 2-5-1 Shikata Cho, Okayama 7008558, Japan Okayama Univ 2-5-1 Shikata Cho Okayama Japan 7008558 , Japan
Citazione:
N. Kobayashi et al., "Successful retroviral gene transfer of simian virus 40 T antigen and herpes simplex virus-thymidine kinase into human hepatocytes", CELL TRANSP, 10(4-5), 2001, pp. 377-381

Abstract

Current clinical reports have indicated that hepatocyte transplantation (HTX) could be used in patients with liver failure and in children with liver-based metabolic diseases. One of the major limiting factors of HTX is a serious shortage of donor livers for hepatocyte isolation. To address this issue, we immortalized adult human hepatocytes with a retroviral vector SSR#69 expressing the genes of simian virus 40 large T antigen and herpes simplex virus-thymidine kinase simultaneously. One of the resulting clones, NKNT-3, grew steadily in chemically defined serum-free medium without any obvious crisis and showed the gene expression of differentiated liver functions. Under the administration of 5 muM ganciclovir, NKNT-3 cells stopped proliferation and died in in vitro experiments. We have established a tightly regulated immortal human hepatocyte cell line. The cells could allow the need for immediate availability of consistent and functionally uniform cells in sufficient quantity and adequate quality.

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Documento generato il 30/11/20 alle ore 12:19:43