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OPAC HELP

Titolo:
Insertion of a suicide gene into an immortalized human hepatocyte cell line
Autore:
Kobayashi, N; Noguchi, H; Totsugawa, T; Watanabe, T; Matsumura, T; Fujiwara, T; Miyazaki, M; Fukaya, K; Namba, M; Tanaka, N;
Indirizzi:
Okayama Univ, Sch Med, Dept Surg 1, Okayama 7008558, Japan Okayama Univ Okayama Japan 7008558 , Dept Surg 1, Okayama 7008558, Japan Okayama Univ, Sch Med, Dept Cell Biol, Okayama 7008558, Japan Okayama Univ Okayama Japan 7008558 ept Cell Biol, Okayama 7008558, Japan
Titolo Testata:
CELL TRANSPLANTATION
fascicolo: 4-5, volume: 10, anno: 2001,
pagine: 373 - 376
SICI:
0963-6897(2001)10:4-5<373:IOASGI>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACUTE LIVER-FAILURE; BIOARTIFICIAL LIVER; ARTIFICIAL LIVER;
Keywords:
immoralized human hepatocytes; suicide gene; herpes simplex virus-thymidine kinase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Kobayashi, N Okayama Univ, Sch Med, Dept Surg 1, 2-5-1 Shikata Cho, Okayama 7008558, Japan Okayama Univ 2-5-1 Shikata Cho Okayama Japan 7008558 , Japan
Citazione:
N. Kobayashi et al., "Insertion of a suicide gene into an immortalized human hepatocyte cell line", CELL TRANSP, 10(4-5), 2001, pp. 373-376

Abstract

For developing a bioartificial liver (BAL) device, an attractive alternative to the primary human hepatocytes would be the use of highly differentiated immortalized human hepatocytes with a safeguard. To test the feasibility, the primary human hepatocytes were immortalized by a plasmid SV3neo encoding simian virus 40 large T antigen (SV40Tag) gene. A highly differentiatedhepatocyte line OUMS-29 was established. A suicide gene of herpes simplex virus-thymidine kinase (HSV-TK) was retrovirally introduced into OUMS-29 cells as a safeguard for clinical application. One of the resulting HSV-TK-positive Cell lines, OUMS-29/tk, grew in chemically defined serum-free mediumwith the gene expression of differentiated liver functions. OUMS-29/tk cells were 100 times more sensitive to ganciclovir compared with unmodified OUMS-29 cells in in vitro experiments. We have established a tightly regulated immortalized human hepatocyte cell line. Essentially unlimited availability of OUMS-29/tk cells may be clinically useful for BAL therapy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 12:08:43