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Titolo:
The activation of PI3-K and PKC zeta in PMA-induced differentiation of HL-60 cells
Autore:
Kim, MS; Lim, WK; Cha, JG; An, NH; Yoo, SJ; Park, JH; Kim, HM; Lee, YM;
Indirizzi:
Wonkwang Univ, Coll Pharm, Iksan 570749, Chonbuk, South Korea Wonkwang Univ Iksan Chonbuk South Korea 570749 0749, Chonbuk, South Korea Wonkwang Univ, Ctr Oriental Med Sci, Iksan 570749, Chonbuk, South Korea Wonkwang Univ Iksan Chonbuk South Korea 570749 0749, Chonbuk, South Korea Pusan Natl Univ, Coll Nat Sci, Dept Mol Biol, Pusan 609735, South Korea Pusan Natl Univ Pusan South Korea 609735 Biol, Pusan 609735, South Korea Wonkwang Univ, Sch Med, Dept Emergency Med, Iksan 570749, Chonbuk, South Korea Wonkwang Univ Iksan Chonbuk South Korea 570749 0749, Chonbuk, South Korea
Titolo Testata:
CANCER LETTERS
fascicolo: 1, volume: 171, anno: 2001,
pagine: 79 - 85
SICI:
0304-3835(20010928)171:1<79:TAOPAP>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; PHOSPHATIDYLINOSITOL 3-KINASE; MONOCYTIC DIFFERENTIATION; MACROPHAGE DIFFERENTIATION; PHOSPHOINOSITIDE 3-KINASE; FAMILY MEMBERS; LEUKEMIA-CELLS; HL60 CELLS; PC12 CELLS; INHIBITOR;
Keywords:
differentiation; phosphoinositide 3-kinase; protein kinase C zeta; HL-60 cell;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Lee, YM Wonkwang Univ, Coll Pharm, Iksan 570749, Chonbuk, South Korea Wonkwang Univ Iksan Chonbuk South Korea 570749 onbuk, South Korea
Citazione:
M.S. Kim et al., "The activation of PI3-K and PKC zeta in PMA-induced differentiation of HL-60 cells", CANCER LETT, 171(1), 2001, pp. 79-85

Abstract

The human myelocytic leukemia cell line HL-60 is a useful model for the study of cellular differentiation. Phorbol 12-myristate 13-acetate (PMA) induces the monocyte/macrophage-like differentiation of HL-60 cells and resultsin growth arrest, increasing adherence. In PMA-induced differentiation of HL-60 cells, phosphoinositide 3-kinase (PI 3-K) activity was measured as phosphatidylinositol(3)P recovery from phosphatidylinositol by in vitro kinase assay. PI 3-K activity was increased in HL-60 cells that were stimulated by 20 nM PMA and the activity was inhibited by pretreatment with 20 muM LY294002, a specific inhibitor of PI 3-K. Members of the protein kinase C (PKC) family have been suggested to be one of the downstream targets of PI 3-K. PKC zeta is one of the atypical PKCs, non-diacylglycerol-responsive PKCs, and the activity was measured by the ability of phosphorylation onto myelinbasic protein. PMA also induced the activation of PKC zeta during monocytic differentiation of HL-60 cells, and LY294002-pretreated cells failed to induce PKC zeta activation. The activity of PI 3-K is essential for PKC activation, and LY294002 blocks both monocytic differentiation of HL-60 cells and activation of PKC zeta during PMA-induced cell differentiation. This implies that activated PI 3-K subsequently stimulates the PKC zeta in the process of PMA-induced monocytic differentiation. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 05:22:11