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Titolo:
Region-specific transcriptional response to chronic nicotine in rat brain
Autore:
Konu, O; Kane, JK; Barrett, T; Vawter, MP; Chang, RY; Ma, JZ; Donovan, DM; Sharp, B; Becker, KG; Li, MD;
Indirizzi:
Univ Tennessee, Dept Pharmacol, Coll Med, Memphis, TN 38163 USA Univ Tennessee Memphis TN USA 38163 acol, Coll Med, Memphis, TN 38163 USA NIDA, NIH, Baltimore, MD 21224 USA NIDA Baltimore MD USA 21224NIDA, NIH, Baltimore, MD 21224 USA Univ Tennessee, Div Biostat, Dept Prevent Med, Coll Med, Memphis, TN 38163USA Univ Tennessee Memphis TN USA 38163 t Med, Coll Med, Memphis, TN 38163USA NIA, NIH, Baltimore, MD 21224 USA NIA Baltimore MD USA 21224NIA, NIH, Baltimore, MD 21224 USA
Titolo Testata:
BRAIN RESEARCH
fascicolo: 1-2, volume: 909, anno: 2001,
pagine: 194 - 203
SICI:
0006-8993(20010803)909:1-2<194:RTRTCN>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE PATHWAY; GENE-EXPRESSION; PHOSPHATIDYLINOSITOL 3-KINASE; TUMOR-SUPPRESSOR; FOS PROTEIN; RECEPTORS; PTEN/MMAC1; DISCOVERY; CANCER; DRUGS;
Keywords:
microarray; normalization; mRNA expression; brain; nicotine; pathway;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Li, MD Univ Tennessee, Dept Pharmacol, Coll Med, 874 Union Ave, Memphis, TN 38163USA Univ Tennessee 874 Union Ave Memphis TN USA 38163 phis, TN 38163USA
Citazione:
O. Konu et al., "Region-specific transcriptional response to chronic nicotine in rat brain", BRAIN RES, 909(1-2), 2001, pp. 194-203

Abstract

Even though nicotine has been shown to modulate mRNA expression of a variety of genes, a comprehensive high-throughput study of the effects of nicotine on the tissue-specific gene expression profiles has been lacking in the literature. In this study, cDNA microarrays containing 1117 genes and ESTs were used to assess the transcriptional response to chronic nicotine treatment in rat, based on four brain regions, Le. prefrontal cortex (PFC), nucleus accumbens (NAs), ventral tegmental area (VTA), and amygdala (AMYG). On the basis of a non-parametric resampling method, an index (called jackknifedreliability index, JRI) was proposed, and employed to determine the inherent measurement error across multiple arrays used in this study. Upon removal of the outliers, the mean correlation coefficient between duplicate measurements increased to 0.978+/-0.0035 from 0.941+/-0.045. Results from principal component analysis and pairwise correlations suggested that brain regions studied were highly similar in terms of their absolute expression levels, but exhibited divergent transcriptional responses to chronic nicotine administration. For example, PFC and NAs were significantly more similar to each other (r=07; p<10(-14-)) than to either VTA or AMYG. Furthermore, we confirmed our microarray results for two representative genes, i.e. the weak inward rectifier K+ channel (TWIK-1), and phosphate and tensin homolog (PTEN) by using real-time quantitative RT-PCR technique. Finally, a number of genes, involved in MAPK, phosphatidylinositol, and EGFR signaling pathways, were identified and proposed as possible targets in response to nicotine administration. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 15/07/20 alle ore 03:54:19