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Titolo:
Nature of the spermatogenic arrest in Dazl -/- mice
Autore:
Schrans-Stassen, BHGJ; Saunders, PTK; Cooke, HJ; de Rooij, DG;
Indirizzi:
Univ Utrecht, Med Ctr, Dept Cell Biol, NL-3584 CX Utrecht, Netherlands Univ Utrecht Utrecht Netherlands NL-3584 CX 3584 CX Utrecht, Netherlands MRC, Human Reprod Sci Unit, Edinburgh EH3 9ET, Midlothian, Scotland MRC Edinburgh Midlothian Scotland EH3 9ET h EH3 9ET, Midlothian, Scotland Western Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland Western Gen Hosp Edinburgh Midlothian Scotland EH4 2XU dlothian, Scotland
Titolo Testata:
BIOLOGY OF REPRODUCTION
fascicolo: 3, volume: 65, anno: 2001,
pagine: 771 - 776
SICI:
0006-3363(200109)65:3<771:NOTSAI>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
VITAMIN-A REPLACEMENT; STEM-CELL RENEWAL; C-KIT RECEPTOR; RAT TESTIS; MALE-INFERTILITY; SEMINIFEROUS EPITHELIUM; INSERTIONAL MUTATION; Y-CHROMOSOME; GENE ENCODES; GERM PLASM;
Keywords:
apoptosis; development; developmental biology; gene regulation; spermatogenesis; testis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: de Rooij, DG Univ Utrecht, Med Ctr, Dept Cell Biol, AZU Rm G02-525,Heidelberglaan 100, NL-3584 CX Utrecht, Netherlands Univ Utrecht AZU Rm G02-525,Heidelberglaan 100 Utrecht Netherlands NL-3584 CX
Citazione:
B.H.G.J. Schrans-Stassen et al., "Nature of the spermatogenic arrest in Dazl -/- mice", BIOL REPROD, 65(3), 2001, pp. 771-776

Abstract

Dazl encodes an RNA-binding protein essential for spermatogenesis. Mice that are deficient for Dazl are infertile, lacking any formation of spermatozoa, and the only germ cells present are spermatogonia and a few spermatocytes. To gain more insight regarding the timing of the spermatogenic arrest in Dazl -/- mice, we studied the spermatogonial cell types present in testissections and in seminiferous tubular whole mounts. Most of the seminiferous tubular cross-sections contained A spermatogonia as the most advanced cell type, with only very few containing cells up to pachytene spermatocytes. Both 5-bromodeoxy-uridine incorporation and mitotic index indicated that the remaining A spermatogonia were actively proliferating. C-kit immunohistochemical studies showed that most of the A spermatogonia were positively stained for the c-Kit protein (similar to 80%). The clonal composition of the A spermatogonia in tubular whole mounts indicated these cells to be A(single) (A(s)), A(paired) (A(pr)), and A(aligned) (A(al)) spermatogonia. It is concluded that the prime spermatogenic defect in the Dazl -/- mice is a failure of the great majority of the A(l), spermatogonia to differentiate into A, spermatogonia. As a result, most seminiferous tubules of Dazl -/- mice only contain actively proliferating A(s), A(pr), and A(al) spermatogonia, with cell production being equaled by apoptosis of these cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 08:03:42