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Titolo:
Crystal structure of the Rac1-RhoGDI complex involved in NADPH oxidase activation
Autore:
Grizot, S; Faure, J; Fieschi, F; Vignais, PV; Dagher, MC; Pebay-Peyroula, E;
Indirizzi:
UJF, Lab BBSI, CNRS,Dept Biol Mol & Struct, CEA Grenoble,UMR 5092, F-38054Grenoble 9, France UJF Grenoble France 9 , CEA Grenoble,UMR 5092, F-38054Grenoble 9, France UJF, Inst Biol Struct, CEA, CNRS,UMR 5075, F-38027 Grenoble, France UJF Grenoble France F-38027 CEA, CNRS,UMR 5075, F-38027 Grenoble, France
Titolo Testata:
BIOCHEMISTRY
fascicolo: 34, volume: 40, anno: 2001,
pagine: 10007 - 10013
SICI:
0006-2960(20010828)40:34<10007:CSOTRC>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
GDP-DISSOCIATION INHIBITOR; GTP-BINDING PROTEIN; RHO-FAMILY; INSERT REGION; EFFECTOR REGION; DOMAIN; RAC; CDC42HS; P67(PHOX); GTPASES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Pebay-Peyroula, E UJF, Lab BBSI, CNRS,Dept Biol Mol & Struct, CEA Grenoble,UMR 5092, 17 Rue des Martyrs, F-38054 Grenoble 9, France UJF 17 Rue des Martyrs Grenoble France 9 oble 9, France
Citazione:
S. Grizot et al., "Crystal structure of the Rac1-RhoGDI complex involved in NADPH oxidase activation", BIOCHEM, 40(34), 2001, pp. 10007-10013

Abstract

A heterodimer of prenylated Rac1 and Rho GDP dissociation inhibitor was purified and found to be competent in NADPH oxidase activation. Small angle neutron scattering experiments confirmed a 1:1 stoichiometry. The crystal structure of the Rac1-RhoGDI complex was determined at 2.7 A resolution. In this complex in which Racl is bound to GDP, the switch I region of Racl is in the GDP conformation whereas the switch II region resembles that of a GTP-bound GTPase. Two types of interaction between RhoGTPases and RhoGDI were investigated. The lipid-protein interaction between the geranylgeranyl moiety of Racl and RhoGDI resulted in numerous structural changes in the core of RhoGDI. The interactions between Racl and RhoGDI occur through hydrogen bonds which involve a number of residues of Racl, namely, Tyr64(Rac), Arg66(Rac), His103(Rac) and His104(Rac), conserved within the Rho family and localized in the switch II region or in its close neighborhood. Moreover, in the switch II region of Racl, hydrophobic interactions involving Leu67(Rac) and Leu70(Rac) contribute to the stability of the Rac1-RhoGDI complex. Inhibition of the GDP-GTP exchange in Racl upon binding to RhoGDI partly resultsfrom interaction of Thr35(Rac) with Asp45(GDI). In the Rac1-RhoGDI complex, the accessibility of the effector loops of Racl probably accounts for theability of the Racl-RhoGDI complex to activate the NADPH oxidase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 05:27:13