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Titolo:
Increased expression of heme oxygenase-1 and bilirubin accumulation in foam cells of rabbit atherosclerotic lesions
Autore:
Nakayama, M; Takahashi, K; Komaru, T; Fukuchi, M; Shioiri, H; Sato, K; Kitamuro, T; Shirato, K; Yamaguchi, T; Suematsu, M; Shibahara, S;
Indirizzi:
Tohoku Univ, Sch Med, Dept Mol Biol & Appl Physiol, Aoba Ku, Sendai, Miyagi 9808575, Japan Tohoku Univ Sendai Miyagi Japan 9808575 Ku, Sendai, Miyagi 9808575, Japan Tohoku Univ, Sch Med, Dept Internal Med 1, Aoba Ku, Sendai, Miyagi 9808575, Japan Tohoku Univ Sendai Miyagi Japan 9808575 Ku, Sendai, Miyagi 9808575, Japan Tokyo Med & Dent Univ, Dept Biochem Genet, Med Res Inst, Tokyo, Japan Tokyo Med & Dent Univ Tokyo Japan hem Genet, Med Res Inst, Tokyo, Japan Keio Univ, Sch Med, Dept Biochem, Tokyo, Japan Keio Univ Tokyo JapanKeio Univ, Sch Med, Dept Biochem, Tokyo, Japan
Titolo Testata:
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
fascicolo: 8, volume: 21, anno: 2001,
pagine: 1373 - 1377
SICI:
1079-5642(200108)21:8<1373:IEOHOA>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY-LIPOPROTEIN; CARBON-MONOXIDE; OXIDATIVE STRESS; MONOCLONAL-ANTIBODY; ENDOTHELIAL-CELLS; OXIDIZED LDL; RAT-LIVER; IN-VIVO; INDUCTION; ANTIOXIDANT;
Keywords:
cholesterol; atherosclerosis; foam cells; heme oxygenase 1; bilirubin IX alpha;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Shibahara, S Tohoku Univ, Sch Med, Dept Mol Biol & Appl Physiol, Aoba Ku, 2-1 Seiryo Machi, Sendai, Miyagi 9808575, Japan Tohoku Univ 2-1 Seiryo Machi Sendai Miyagi Japan 9808575 apan
Citazione:
M. Nakayama et al., "Increased expression of heme oxygenase-1 and bilirubin accumulation in foam cells of rabbit atherosclerotic lesions", ART THROM V, 21(8), 2001, pp. 1373-1377

Abstract

Heme oxygenase-1 (HO-1) catalyzes the regiospecific oxidative degradation of heme to biliverdin IX alpha, iron, and carbon monoxide. Biliverdin IX alpha is subsequently reduced to bilirubin IX alpha by biliverdin reductase. HO-1 expression is induced under various disease conditions, including atherosclerosis, but it is unknown whether HO-1 catalyzes heme breakdown in theregions at risk. Using hypercholesterolemic rabbits fed a cholesterol-enriched diet, we attempted to demonstrate the involvement of HO-1 induction and bilirubin IX alpha production in atherosclerotic regions. Expression levels of HO-1 mRNA were elevated in the aortas of hypercholesterolemic rabbits. In situ hybridization and immunohistochemistry revealed that mRNA and protein of HO-1 are induced in endothelial cells and foam cells (lipid-filled macrophages) in atherosclerotic lesions. Furthermore, immunohistochemistry with the use of an anti-bilirubin-IX alpha monoclonal antibody, 24G7, demonstrated accumulation of bilirubin IX alpha in foam cells, indicating that heme is actually degraded in atherosclerotic lesions. Remarkably, bilirubin IX alpha, like HO-1 protein, is predominantly accumulated in the perinuclear regions of foam cells. These results provide the first in vivo evidence of the colocalization of HO-1 and bilirubin IX alpha in foam cells, suggesting a role of HO-1 induction in the modulation of macrophage activation in atherosclerosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/06/20 alle ore 01:54:04