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Titolo:
Arterial elastase activity after balloon angioplasty and effects of elafin, an elastase inhibitor
Autore:
Barolet, AW; Nili, N; Cheema, A; Robinson, R; Natarajan, MK; OBlenes, S; Li, J; Eskandarian, MR; Sparkes, J; Rabinovitch, M; Strauss, BH;
Indirizzi:
St Michaels Hosp, Terrence Donnelly Heart Ctr, Div Cardiol, Toronto, ON M5B 1W8, Canada St Michaels Hosp Toronto ON Canada M5B 1W8 l, Toronto, ON M5B 1W8, Canada Hosp Sick Children, Res Inst, Div Cardiovasc Res, Toronto, ON M5G 1X8, Canada Hosp Sick Children Toronto ON Canada M5G 1X8 Toronto, ON M5G 1X8, Canada Univ Toronto, Mt Sinai Hosp, Div Cardiol, Toronto, ON M5G 1X5, Canada UnivToronto Toronto ON Canada M5G 1X5 rdiol, Toronto, ON M5G 1X5, Canada Univ Toronto, Dept Med, Toronto, ON, Canada Univ Toronto Toronto ON Canada iv Toronto, Dept Med, Toronto, ON, Canada Univ Toronto, Dept Pediat, Toronto, ON, Canada Univ Toronto Toronto ON Canada Toronto, Dept Pediat, Toronto, ON, Canada Univ Toronto, Dept Pathol, Toronto, ON, Canada Univ Toronto Toronto ON Canada Toronto, Dept Pathol, Toronto, ON, Canada
Titolo Testata:
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
fascicolo: 8, volume: 21, anno: 2001,
pagine: 1269 - 1274
SICI:
1079-5642(200108)21:8<1269:AEAABA>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; SERINE ELASTASE; PULMONARY-HYPERTENSION; EXTRACELLULAR-MATRIX; PROTEINASE-INHIBITOR; ELASTOLYTIC ACTIVITY; VASCULAR-DISEASE; HUMAN SKIN; INJURY; RATS;
Keywords:
angioplasty; restenosis; elastase; elafin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Strauss, BH St Michaels Hosp, Terrence Donnelly Heart Ctr, Div Cardiol, 30Bond St, Toronto, ON M5B 1W8, Canada St Michaels Hosp 30 Bond St Toronto ON Canada M5B 1W8 , Canada
Citazione:
A.W. Barolet et al., "Arterial elastase activity after balloon angioplasty and effects of elafin, an elastase inhibitor", ART THROM V, 21(8), 2001, pp. 1269-1274

Abstract

Increased proteolytic activity may be a factor in intimal hyperplasia after balloon angioplasty (BA). The objectives of this study were to assess elastase activity after BA in a rabbit arterial double-injury model and the effects of elastase inhibition. Elastase activity increased immediately afterBA, reached an 8-fold peak at 1 week, and declined to baseline levels by 4weeks. Elastin zymography showed that the elastase activity was associatedpredominantly with a molecular mass of 25 kDa. Elastase activity was significantly inhibited in vitro by elafin and phenymethylsulfonyl fluoride, selective inhibitors of serine elastases. A second group of animals was transfected after BA with a plasmid containing the cDNA for either elafin or a control (chloramphenicol acetyltransferase, CAT) construct by using a hemagglutinating virus of Japan-liposome transfection technique. Arterial segmentswere obtained at 48 hours, 1 week, and 4 weeks to assess transgene expression, arterial wall elastase activity, and intimal. cross-sectional area, respectively. Elafin transgene expression was evident at 48 hours and resulted in a significant (80%) inhibition of elastase activity compared with chloramphenicol acetyltransferase-transfected arteries. There was a 43% reduction in intimal cross-sectional area in elafin-transfected arteries (0.28 +/-0.22 versus 0.16 +/-0.07 mm(2) for CAT-transfected versus elafin-transfected arteries, respectively; P <0.05). These data suggest that an early increase in serine elastase activity after BA contributes to intimal hyperplasia. Serine elastase inhibition may be a potential therapeutic approach to inhibit intimal hyperplasia.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 10:55:46