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Titolo:
Caveolar and intercellular channels provide major transport pathways of macromolecules across vascular endothelial cells
Autore:
Ogawa, K; Imai, M; Ogawa, T; Tsukamoto, Y; Sasaki, F;
Indirizzi:
Univ Osaka Prefecture, Grad Sch Agr & Biol Sci, Dept Vet Anat, Osaka 5998531, Japan Univ Osaka Prefecture Osaka Japan 5998531 Vet Anat, Osaka 5998531, Japan Natl Inst Infect Dis, Dept Virol 1, Tokyo, Japan Natl Inst Infect Dis Tokyo Japan Infect Dis, Dept Virol 1, Tokyo, Japan Iwate Med Univ, Sch Med, Dept Pathol, Morioka, Iwate, Japan Iwate Med Univ Morioka Iwate Japan d, Dept Pathol, Morioka, Iwate, Japan Iwate Univ, Fac Agr, Dept Vet Anat, Morioka, Iwate 0208550, Japan Iwate Univ Morioka Iwate Japan 0208550 nat, Morioka, Iwate 0208550, Japan
Titolo Testata:
ANATOMICAL RECORD
fascicolo: 1, volume: 264, anno: 2001,
pagine: 32 - 42
SICI:
0003-276X(20010901)264:1<32:CAICPM>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMALEMMAL VESICULAR SYSTEM; 3-DIMENSIONAL ORGANIZATION; MICROVASCULAR PERMEABILITY; CAPILLARY ENDOTHELIUM; MUSCLE CAPILLARIES; AORTIC ENDOTHELIUM; N-ETHYLMALEIMIDE; SERIAL SECTIONS; RETE MIRABILE; VESICLES;
Keywords:
caveolae; channels; endothelium; NEM; transport;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Ogawa, K Univ Osaka Prefecture, Grad Sch Agr & Biol Sci, Dept Vet Anat, 1-1 Gakuen Cho, Osaka 5998531, Japan Univ Osaka Prefecture 1-1 Gakuen Cho Osaka Japan 5998531 , Japan
Citazione:
K. Ogawa et al., "Caveolar and intercellular channels provide major transport pathways of macromolecules across vascular endothelial cells", ANAT REC, 264(1), 2001, pp. 32-42

Abstract

Serum macromolecules are transported through the vascular endothelial layer to the interstitium via the caveolae and interendothelial clefts, but thenature of the permeability of these structures is unknown, and the manner of caveola-vesicle transport is controversial. We have developed a method of detecting macromolecular channels using an in situ HRP perfusion into arteries previously perfused with aldehyde and random conventional sectioning for electron microscopy. Using unbiased morphometry, 4.75% of the abluminalcaveolae and 15.13% of the intercellular clefts were the tracer-positive in rat aortic endothelium. In rat aortas treated with N-ethylmaleimide, all caveolae and most free vesicles in the cytoplasm except those around the Golgi area were HRP-positive in the endothelial cells; 1.48% of abluminal caveolae were structurally recognized as caveolar channels through the endothelial layer in a plane of single section. The length density of the abluminal caveolae was decreased to about 80% to the physiological control level whereas the larger invaginations were more frequently observed. Moreover 96.17% of the intercellular clefts were HRP-positive. We suggest that a flexible channel-system functions extensively as a macromolecular transport pathway in the arterial endothelium in vivo because the tracer-labeled abluminal caveolae and intercellular clefts should be opened to the luminal surfaces methodologically. We therefore propose that caveolar channels, rather than transcytosis, provide a mechanism of caveola-vesicle transport in the endothelial cells, because free vesicles involved in transcytosis were few in number. Anat Rec 264:32-42, 2001. (C) 2001 Wiley-Liss, Inc.

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Documento generato il 04/12/20 alle ore 13:10:10