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Titolo:
Fluorimetric determination of total ascorbic acid by a stopped-now mixing technique
Autore:
Perez-Ruiz, T; Martinez-Lozano, C; Tomas, V; Fenol, J;
Indirizzi:
Univ Murcia, Fac Chem, Dept Analyt Chem, E-30071 Murcia, Spain Univ Murcia Murcia Spain E-30071 Dept Analyt Chem, E-30071 Murcia, Spain
Titolo Testata:
ANALYST
fascicolo: 8, volume: 126, anno: 2001,
pagine: 1436 - 1439
SICI:
0003-2654(200108)126:8<1436:FDOTAA>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLUOROMETRIC-DETERMINATION; BIOLOGICAL SAMPLES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Perez-Ruiz, T Univ Murcia, Fac Chem, Dept Analyt Chem, E-30071 Murcia, Spain Univ Murcia Murcia Spain E-30071 em, E-30071 Murcia, Spain
Citazione:
T. Perez-Ruiz et al., "Fluorimetric determination of total ascorbic acid by a stopped-now mixing technique", ANALYST, 126(8), 2001, pp. 1436-1439

Abstract

A simple, rapid and automatic fluorimetric method for the determination oftotal ascorbic acid is described. The method makes use of the stopped-flowmixing technique in order to achieve the rapid oxidation of ascorbic acid by dissolved oxygen to dehydroascorbic acid, which then reacts with o-phenylenediamine to form a fluorescent quinoxaline. The initial rate and fluorescence signal of this system are directly proportional to the ascorbic acid concentration. The calibration graph was linear over the range 0.1-30 mug ml(-1) (kinetic method) and 0.25-34 mug ml(-1) (equilibrium method). The precision (% RSD) was close to 0.5%. The method has been used for the determination of ascorbic acid in pharmaceutical formulations, fruit juices, soft drinks and blood serum.

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Documento generato il 27/05/20 alle ore 15:39:18