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Titolo:
Persistent mucin glycoprotein alterations in equine recurrent airway obstruction
Autore:
Jefcoat, AM; Hotchkiss, JA; Gerber, V; Harkema, JR; Basbaum, CB; Robinson, NE;
Indirizzi:
Michigan State Univ, Coll Vet Med, Dept Large Anim Clin Sci, E Lansing, MI48824 USA Michigan State Univ E Lansing MI USA 48824 in Sci, E Lansing, MI48824 USA Michigan State Univ, Coll Vet Med, Dept Vet Pathol, E Lansing, MI 48824 USA Michigan State Univ E Lansing MI USA 48824 athol, E Lansing, MI 48824 USA Univ Calif San Francisco, Sch Med, Dept Anat, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
fascicolo: 3, volume: 281, anno: 2001,
pagine: L704 - L712
SICI:
1040-0605(200109)281:3<L704:PMGAIE>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
PULMONARY-DISEASE; MONOCLONAL-ANTIBODIES; HORSES; ANTIGENS; PATHOGENESIS; HEAVES; MUCUS;
Keywords:
bronchoalveolar lavage fluid; O-linked mucin glycoproteins; oligosaccharide side chains; lectins;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Jefcoat, AM Michigan State Univ, Natl Food Safety & Toxicol Ctr 210, E Lansing, MI 48824 USA Michigan State Univ E Lansing MI USA 48824 sing, MI 48824 USA
Citazione:
A.M. Jefcoat et al., "Persistent mucin glycoprotein alterations in equine recurrent airway obstruction", AM J P-LUNG, 281(3), 2001, pp. L704-L712

Abstract

Horses with the episodic asthmalike condition of recurrent airway obstruction (RAO) have bouts of inflammation and bronchoconstriction. associated with indoor housing. To assess the potential differences in airway secretionsbetween RAO-affected and control horses, methods to quantify mucus secretions were developed and applied to bronchoalveolar lavage fluid. The relative difference in the amount of mucin glycoproteins between control and RAO-affected horses was assessed with a carbohydrate side chain-specific monoclonal antibody (4E4) in an enzyme-linked immunosorbent assay and by carbohydrate-specific enzyme-linked lectin assays. Significantly increased levels of4E4-immunoreactive glycoprotein and the mucin-associated carbohydrates fucose (alpha -1,2 linkage) and N-acetylglucosamine were detected in RAO-affected horses in acute disease. RAO-affected horses in remission maintained significantly elevated levels of alpha -1,2-fucose and N-acetylglucosamine, whereas the 4E4-immunoreactive glycoprotein levels displayed a trend toward an increase over control levels. These results indicated that persistent changes in the quantity and/or quality of mucus glycoproteins occurred in theRAO-affected horses.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 22:05:07