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Titolo:
Surfactant proteins A and D in Eustachian tube epithelium
Autore:
Paananen, R; Sormunen, R; Glumoff, V; Van Eijk, M; Hallman, M;
Indirizzi:
Univ Oulu, Dept Pediat, FIN-90014 Oulu, Finland Univ Oulu Oulu Finland FIN-90014 u, Dept Pediat, FIN-90014 Oulu, Finland Univ Oulu, Bioctr, FIN-90014 Oulu, Finland Univ Oulu Oulu Finland FIN-90014 v Oulu, Bioctr, FIN-90014 Oulu, Finland Univ Oulu, Dept Pathol, FIN-90014 Oulu, Finland Univ Oulu Oulu Finland FIN-90014 u, Dept Pathol, FIN-90014 Oulu, Finland Univ Utrecht, Fac Vet Med, Dept Biochem & Cell Biol, NL-3508 TD Utrecht, Netherlands Univ Utrecht Utrecht Netherlands NL-3508 TD 3508 TD Utrecht, Netherlands
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
fascicolo: 3, volume: 281, anno: 2001,
pagine: L660 - L667
SICI:
1040-0605(200109)281:3<L660:SPAADI>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESPIRATORY SYNCYTIAL VIRUS; SP-A; PULMONARY SURFACTANT; DEVELOPMENTAL EXPRESSION; PSEUDOMONAS-AERUGINOSA; ALVEOLAR MACROPHAGES; OTITIS-MEDIA; MIDDLE-EAR; II CELLS; IN-VIVO;
Keywords:
collectin; innate immunity; in situ hybridization; immunoelectron microscopy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Paananen, R Univ Oulu, Dept Pediat, FIN-90014 Oulu, Finland Univ Oulu Oulu Finland FIN-90014 at, FIN-90014 Oulu, Finland
Citazione:
R. Paananen et al., "Surfactant proteins A and D in Eustachian tube epithelium", AM J P-LUNG, 281(3), 2001, pp. L660-L667

Abstract

Surfactant protein (SP) A and SP-D are collectins that have roles in host defense. The Eustachian tube (ET) maintains the patency between the upper airways and the middle ear. Dysfunction of local mucosal immunity in ET may predispose infants to recurrent otitis media. We recently described preliminary evidence of the expression of SP-A and SP-D in the ET. Our present aimwas to establish the sites of SP-A and SP-D expression within the epithelium of the ET in vivo. With in situ hybridization, electron microscopy, and immunoelectron microscopy, the cells responsible for SP-A and SP-D expression and storage were identified. SP-A expression was localized within the ETepithelium, and the protein was found in the electron-dense granules of microvillar epithelial cells. Being concentrated in the epithelial lining, only a few cells revealed intracellular SP-D, and it was not associated with granules. The SP-A and SP-D immunoreactivities in ET lavage fluid, as shownby Western blot analyses, were similar to those in bronchoalveolar lavage fluid. We propose that there are specialized cells in the ET epithelium expressing and secreting SP-A and SP-D. SP-A and SP-D may be important for antibody-independent protection of the middle ear against infections.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 07:09:45