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Titolo:
EGF receptor function is required in late G(1) for cell cycle progression induced by bombesin and bradykinin
Autore:
Santiskulvong, C; Sinnett-Smith, J; Rozengurt, E;
Indirizzi:
Univ Calif Los Angeles, Sch Med, Dept Med, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA Univ Calif Los Angeles, Inst Mol Biol, Los Angeles, CA 90095 USA Univ Calif Los Angeles Los Angeles CA USA 90095 Los Angeles, CA 90095 USA
Titolo Testata:
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
fascicolo: 3, volume: 281, anno: 2001,
pagine: C886 - C898
SICI:
0363-6143(200109)281:3<C886:ERFIRI>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
SWISS 3T3 CELLS; GROWTH-FACTOR RECEPTOR; ACTIVATED PROTEIN-KINASE; FOCAL ADHESION KINASE; SIGNAL-TRANSDUCTION PATHWAYS; TYROSINE KINASE; COUPLED RECEPTORS; DNA-SYNTHESIS; LYSOPHOSPHATIDIC ACID; PEPTIDE RECEPTOR;
Keywords:
epidermal growth factor receptor transactivation; tyrphostin AG-1478; G protein-coupled receptors; Swiss 3T3 cells; mitogen-activated protein kinases;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
65
Recensione:
Indirizzi per estratti:
Indirizzo: Rozengurt, E Univ Calif Los Angeles, Sch Med, Dept Med, 900 Vet Ave,WarrenHall,Rm 11-124, Los Angeles, CA 90095 USA Univ Calif Los Angeles 900 Vet Ave,Warren Hall,Rm 11-124 Los Angeles CA USA 90095
Citazione:
C. Santiskulvong et al., "EGF receptor function is required in late G(1) for cell cycle progression induced by bombesin and bradykinin", AM J P-CELL, 281(3), 2001, pp. C886-C898

Abstract

We examined the role of epidermal growth factor (EGF) receptor (EGFR) tyrosine kinase activation in G protein-coupled receptor (GPCR) agonist-inducedmitogenesis in Swiss 3T3 and Rat-1 cells. Addition of EGFR tyrosine kinaseinhibitors (e.g., tyrphostin AG-1478) abrogated bombesin-induced extracellular signal-regulated kinase (ERK) activation in Rat-1 cells but not in Swiss 3T3 cells, indicating the importance of cell context in determining the role of EGFR in ERK activation. In striking contrast, treatment with tyrphostin AG-1478 markedly (similar to 70%) inhibited DNA synthesis induced by bombesin in both Swiss 3T3 and Rat-1 cells. Similar inhibition of bombesin-induced DNA synthesis in Swiss 3T3 cells was obtained using four structurally different inhibitors of EGFR tyrosine kinase. Furthermore, kinetic analysis indicates that EGFR function is necessary for bombesin-induced mitogenesis in mid-late G(1) in both Swiss 3T3 and Rat-1 cells. Our results indicatethat EGFR kinase activity is necessary in mid-late G(1) for promoting the accumulation of cyclins D1 and E and implicate EGFR function in the coupling of GPCR signaling to the activation of the cell cycle.

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Documento generato il 25/11/20 alle ore 18:45:52