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Titolo:
In vitro study of prebiotic properties of Levan-type exopolysaccharides from lactobacilli and non-digestible carbohydrates using denaturing gradient gel electrophoresis
Autore:
Dal Bello, F; Walter, J; Hertel, C; Hammes, WP;
Indirizzi:
Univ Hohenheim, Inst Food Technol, D-70599 Stuttgart, Germany Univ Hohenheim Stuttgart Germany D-70599 nol, D-70599 Stuttgart, Germany
Titolo Testata:
SYSTEMATIC AND APPLIED MICROBIOLOGY
fascicolo: 2, volume: 24, anno: 2001,
pagine: 232 - 237
SICI:
0723-2020(200107)24:2<232:IVSOPP>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
LACTIC-ACID BACTERIA; FECAL SAMPLES; CONTINUOUS-CULTURE; BIFIDOBACTERIA; OLIGOFRUCTOSE;
Keywords:
exopolysaccharide; Lactobacillas sanfranciscensis; fructooligosaccharide; DGGE; prebiotics; intestinal flora;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Hertel, C Univ Hohenheim, Inst Food Technol, Garbenstr 28, D-70599 Stuttgart, Germany Univ Hohenheim Garbenstr 28 Stuttgart Germany D-70599 , Germany
Citazione:
F. Dal Bello et al., "In vitro study of prebiotic properties of Levan-type exopolysaccharides from lactobacilli and non-digestible carbohydrates using denaturing gradient gel electrophoresis", SYST APPL M, 24(2), 2001, pp. 232-237

Abstract

Batch cultures inoculated with human faeces were used to study the prebiotic properties of levan-type exopolysaccharides (EPS) from Lactobacillus sanfranciscensis as well as levan, inulin, and fructooligosaccharide (FOS). Denaturing gradient gel electrophoresis of 16S rDNA fragments generated by PCR with universal primers was used to analyse the cultures. Characteristic changes were revealed in the composition of the gut bacteria during fermentation of the carbohydrates. An enrichment of Bifidobacterium spp. was found for the EPS and inulin but not for levan and FOS. The bifidogenic effect ofthe EPS was confirmed by culturing on selective medium. In addition, the use of EPS and FOS resulted in enhanced growth of Eubacterium biforme and Clostridium perfringens, respectively.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 07:50:58