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Titolo:
Manipulating biological samples for environmental scanning electron microscopy observation
Autore:
Tai, SSW; Tang, XM;
Indirizzi:
Shanghai Med Univ 2, Dept Biophys & Cell Biol, Shanghai 200025, Peoples R China Shanghai Med Univ 2 Shanghai Peoples R China 200025 025, Peoples R China
Titolo Testata:
SCANNING
fascicolo: 4, volume: 23, anno: 2001,
pagine: 267 - 272
SICI:
0161-0457(200107/08)23:4<267:MBSFES>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
STAIN REMOVAL; SEM;
Keywords:
environmental scanning electron microscopy; biological samples;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Tang, XM Shanghai Med Univ 2, Dept Biophys & Cell Biol, 280 S Chongqing Rd, Shanghai 200025, Peoples R China Shanghai Med Univ 2 280 S Chongqing Rd Shanghai Peoples R China 200025
Citazione:
S.S.W. Tai e X.M. Tang, "Manipulating biological samples for environmental scanning electron microscopy observation", SCANNING, 23(4), 2001, pp. 267-272

Abstract

Biological samples having different characteristics were observed by environmental scanning electron microscopy (ESEM). The environmental conditions for untreated biological samples was determined by optimizing sample temperature and chamber pressure. When the temperature was at 4 degrees -6 degreesC and chamber pressure was 5.2-5.9 Torr, the relative humidity in the specimen chamber was about 85%. Under these conditions, the surface features ofthe sample were completely exposed and did not exhibit charging. The images obtained from the untreated samples at different ESEM conditions were also compared with fixed and coated samples observed under high vacuum.

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Documento generato il 05/04/20 alle ore 06:51:11