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Titolo:
Inhibition of neutrophil responses by cyclosporin A. An insight into molecular mechanisms
Autore:
Spisani, S; Fabbri, E; Muccinelli, M; Cariani, A; Barbin, L; Trotta, F; Dovigo, L;
Indirizzi:
Arcispedale St Anna, Div Rheumatol, I-44100 Ferrara, Italy Arcispedale St Anna Ferrara Italy I-44100 umatol, I-44100 Ferrara, Italy Univ Ferrara, Dept Biochem & Mol Biol, I-44100 Ferrara, Italy Univ Ferrara Ferrara Italy I-44100 em & Mol Biol, I-44100 Ferrara, Italy Univ Ferrara, Dept Biol, Sect Gen Physiol, I-44100 Ferrara, Italy Univ Ferrara Ferrara Italy I-44100 t Gen Physiol, I-44100 Ferrara, Italy Univ Bologna, Dept Biol, I-40126 Bologna, Italy Univ Bologna Bologna Italy I-40126 na, Dept Biol, I-40126 Bologna, Italy
Titolo Testata:
RHEUMATOLOGY
fascicolo: 7, volume: 40, anno: 2001,
pagine: 794 - 800
SICI:
1462-0324(200107)40:7<794:IONRBC>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
RHEUMATOID-ARTHRITIS; PERIPHERAL-BLOOD; SYNOVIAL-FLUID; DERIVATIVES; GENERATION; INVIVO;
Keywords:
CsA; rheumatoid arthritis; neutrophil functionality; cytosolic calcium; receptor binding assay;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Dovigo, L Arcispedale St Anna, Div Rheumatol, Cso Giovecca 203, I-44100 Ferrara, Italy Arcispedale St Anna Cso Giovecca 203 Ferrara Italy I-44100 taly
Citazione:
S. Spisani et al., "Inhibition of neutrophil responses by cyclosporin A. An insight into molecular mechanisms", RHEUMATOLOG, 40(7), 2001, pp. 794-800

Abstract

Objective. Cyclosporin A (CsA) is an effective agent in rheumatoid arthritis (RA), slowing joint damage progression. Its therapeutic effect on T lymphocytes has been studied extensively, but there is little information available about neutrophils, the cells responsible for a substantial proportion of inflammation. A study was performed to investigate the in vitro effects of CsA on neutrophil functions triggered by several agonists and determine whether the drug could counteract the binding of formyl-methionyl-leucyl-phenylalanine (fMLP) to its receptor and or modulate changes in the intracellular Ca2+ concentration ([Ca2+](i)). Methods. CsA was added to neutrophils 5-50 min before the incubation stepsfor neutrophil function assays (chemotaxis, superoxide anion production, lysozyme release), calcium measurements and receptor binding experiments. Results. CsA appeared to be particularly effective in lowering chemotaxis,Superoxide anion production and lysozyme release induced by different agonists. However, it did not significantly affect either basal or agonist-stimulated neutrophil [Ca2+](i) and the interaction between fMLP and its receptor. Conclusions. Because of its in vitro inhibition of neutrophil functions, CsA appears to have considerable potential as an anti-inflammatory drug. Moreover, as it is also a potent immunosuppressive agent, it may reduce the progression of joint damage in RA. More work remains to be done to clarify the molecular mechanism of CsA action on neutrophils.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 06:29:15