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Titolo:
Monoclonal antibodies against human ribosomal P proteins penetrate into living cells and cause apoptosis of Jurkat T cells in culture
Autore:
Sun, KH; Tang, SJ; Lin, ML; Wang, YS; Sun, GH; Liu, WT;
Indirizzi:
Natl Yang Ming Univ, Fac Med Technol, Taipei 112, Taiwan Natl Yang Ming Univ Taipei Taiwan 112 ac Med Technol, Taipei 112, Taiwan Natl Yang Ming Univ, Inst Biotechnol Med, Taipei 112, Taiwan Natl Yang Ming Univ Taipei Taiwan 112 Biotechnol Med, Taipei 112, Taiwan Natl Taiwan Ocean Univ, Natl Marine Biotechnol, Taipei, Taiwan Natl TaiwanOcean Univ Taipei Taiwan Marine Biotechnol, Taipei, Taiwan TriServ Gen Hosp, Dept Surg, Div Urol, Taipei, Taiwan TriServ Gen Hosp Taipei Taiwan osp, Dept Surg, Div Urol, Taipei, Taiwan Natl Def Med Ctr, Taipei, Taiwan Natl Def Med Ctr Taipei TaiwanNatl Def Med Ctr, Taipei, Taiwan
Titolo Testata:
RHEUMATOLOGY
fascicolo: 7, volume: 40, anno: 2001,
pagine: 750 - 756
SICI:
1462-0324(200107)40:7<750:MAAHRP>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYSTEMIC LUPUS-ERYTHEMATOSUS; VEIN ENDOTHELIAL-CELLS; CELLULAR DYSFUNCTION; AUTOANTIBODIES; SURFACE; MEMBRANE; REACT; ASSOCIATION; LYMPHOCYTES; INHIBITION;
Keywords:
anti-P autoantibody; T lymphocytes; apoptosis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Sun, KH Natl Yang Ming Univ, Fac Med Technol, 155 Sect 2,Lie Nong St, Taipei 112, Taiwan Natl Yang Ming Univ 155 Sect 2,Lie Nong St Taipei Taiwan 112 iwan
Citazione:
K.H. Sun et al., "Monoclonal antibodies against human ribosomal P proteins penetrate into living cells and cause apoptosis of Jurkat T cells in culture", RHEUMATOLOG, 40(7), 2001, pp. 750-756

Abstract

Objective. This study was designed to determine the role or autoantibodiesto the ribosomal P protein (anti-P Abs) in the pathogenesis of systemic lupus erythematosus (SLE) using monoclonal anti-P antibodies (anti-P mAbs). Methods. Anti-P mAbs were prepared by a standard hybridoma procedure usingrecombinant human P1 and P2 proteins as immunogens. We studied the reactivities of these mAbs to P proteins, their binding and penetration capabilities in different cell lines and their apoptotic effects on Jurkat T cells. Results. In addition to recognizing human P0, P1 and P2 proteins, the anti-P mAb 9B6-4 bound to 20-40% and penetrated 50-90% of astrocytes, Jurkat T cells and lung cancer cells via the P0 surface protein. Treatment with the mAb 9B6-4 also caused increases in the percentages of Jurkat T cells in thesub-G1 phase of the cell cycle (14.8%,) and undergoing apoptosis (21.3%). Conclusion. Anti-P autoantibodies may play a role in the pathogenesis of lymphopenia or lymphocyte dysfunction in SLE.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 12:05:15