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Titolo:
Partial complementation of embryo defective mutations: a general strategy to elucidate gene function
Autore:
Despres, B; Delseny, M; Devic, M;
Indirizzi:
Univ Perpignan, Lab Genome & Dev Plantes, CNRS, UMR 5096, F-66860 Perpignan, France Univ Perpignan Perpignan France F-66860 5096, F-66860 Perpignan, France
Titolo Testata:
PLANT JOURNAL
fascicolo: 2, volume: 27, anno: 2001,
pagine: 149 - 159
SICI:
0960-7412(200107)27:2<149:PCOEDM>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARABIDOPSIS-THALIANA; ANKYRIN REPEAT; ABSCISIC-ACID; MUTANTS; PROTEIN; CHLOROPLAST; EXPRESSION; ENCODES; MAIZE; RNA;
Keywords:
embryogenesis; chloroplast; ABl3; mutant; complementation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Devic, M Univ Perpignan, Lab Genome & Dev Plantes, CNRS, UMR 5096, 52 Ave Villeneuve, F-66860 Perpignan, France Univ Perpignan 52 Ave Villeneuve Perpignan France F-66860 France
Citazione:
B. Despres et al., "Partial complementation of embryo defective mutations: a general strategy to elucidate gene function", PLANT J, 27(2), 2001, pp. 149-159

Abstract

The EMB 506 gene has been characterised as essential for embryo development. To provide insights into the role of EMB 506, which is hidden by the embryo defective phenotype, the ABI3 promoter was fused to the EMB 506 cDNA. The expression of such a transgene should provide sufficient protein during embryogenesis to ensure normal embryo development in homozygous emb 506 seeds. We show that homozygous emb 506 seedlings, partially complemented with the ABI3::EMB 506 transgene, can be obtained. Most of the rescued emb 506 plants are able to flower and to set normal seeds, but show mild to severe depigmentation of rosette leaves and/or inflorescences. This effect on chloroplast development indicated a putative chloroplast localisation of the EMB506 protein, which was demonstrated by GFP-protein fusion. However, EMB 506 cannot be considered as a chloroplast housekeeping protein only, since EMB 506 is not present in all photosynthetic tissues. This study demonstratesthe power of this simple strategy, which could be widely applied to other emb mutants and which may reveal similar or additional roles for EMB genes at vegetative stages of the life cycle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 22:47:59