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Titolo:
Evidence for a disease-resistance pathway in rice similar to the NPR1-mediated signaling pathway in Arabidopsis
Autore:
Chern, MS; Fitzgerald, HA; Yadav, RC; Canlas, PE; Dong, XN; Ronald, PC;
Indirizzi:
Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA Univ Calif DavisDavis CA USA 95616 ept Plant Pathol, Davis, CA 95616 USA Duke Univ, Dept Biol, Dev Cell & Mol Biol Grp, Durham, NC 27708 USA Duke Univ Durham NC USA 27708 v Cell & Mol Biol Grp, Durham, NC 27708 USA
Titolo Testata:
PLANT JOURNAL
fascicolo: 2, volume: 27, anno: 2001,
pagine: 101 - 113
SICI:
0960-7412(200107)27:2<101:EFADPI>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYSTEMIC ACQUIRED-RESISTANCE; SALICYLIC-ACID; LEUCINE-ZIPPER; TRANSCRIPTION FACTORS; INDUCIBLE EXPRESSION; GENE-EXPRESSION; DNA-BINDING; PROTEIN; MUTANT; BENZOTHIADIAZOLE;
Keywords:
rice; Xanthomonas oryzae; SAR; NPR1; bZIP; liguleless;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Ronald, PC Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA Univ Calif Davis Davis CA USA 95616 athol, Davis, CA 95616 USA
Citazione:
M.S. Chern et al., "Evidence for a disease-resistance pathway in rice similar to the NPR1-mediated signaling pathway in Arabidopsis", PLANT J, 27(2), 2001, pp. 101-113

Abstract

The Arabidopsis NPR1/NIM1 gene is a key regulator of systemic acquired resistance (SAR). Overexpression of NPR1 leads to enhanced resistance in Arabidopsis. To investigate the role of NPR1 in monocots, we over-expressed the Arabidopsis NPR1 in rice and challenged the transgenic plants with Xanthomonas oryzae pv. oryzae (Xoo), the rice bacteria] blight pathogen. The transgenic plants displayed enhanced resistance to Xoo. RNA blot hybridization indicates that enhanced resistance requires expression of NPR1 mRNA above a threshold level in rice. To identify components mediating the resistance controlled by NPR1, we used NPR1 as bait in a yeast two-hybrid screen. We isolated four cDNA clones encoding rice NPR1 interactors (named rTGA2.1, rTGA2.2, rTGA2.3 and rLG2) belonging to the bZIP family. rTGA2.1, rTGA2.2 and rTGA2.3 share 75, 76 and 78% identity with Arabidopsis TGA2, respectively. In contrast, rLG2 shares highest identity (81%) to the maize liguleless (LG2) gene product, which is involved in establishing the leaf blade-sheath boundary. The interaction of NPR1 with the rice bZIP proteins in yeast was impaired by the npr1-1 and npr1-2 mutations, but not by the nim1-4 mutation. TheNPR1-rTGA2.1 interaction was confirmed by an in vitro pull-down experiment. In gel mobility shift assays, rTGA2.1 binds to the rice RCH10 promoter and to a cis-element required sequence-specifically for salicylic acid responsiveness. This is the first demonstration that the Arabidopsis NPR1 gene can enhance disease resistance in a monocot plant. These results also suggestthat monocot and dicot plants share a conserved signal transduction pathway controlling NPR1-mediated resistance.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 19:02:38