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Titolo:
A fast neutron deletion mutagenesis-based reverse genetics system for plants
Autore:
Li, X; Song, YJ; Century, K; Straight, S; Ronald, P; Dong, XN; Lassner, M; Zhang, YL;
Indirizzi:
Maxygen Davis, Davis, CA 95616 USA Maxygen Davis Davis CA USA 95616Maxygen Davis, Davis, CA 95616 USA Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA Univ Calif DavisDavis CA USA 95616 ept Plant Pathol, Davis, CA 95616 USA Duke Univ, Dept Biol, Dev Cell & Mol Biol Grp, Durham, NC 27708 USA Duke Univ Durham NC USA 27708 v Cell & Mol Biol Grp, Durham, NC 27708 USA Maxygen Inc, Redwood City, CA 94063 USA Maxygen Inc Redwood City CA USA 94063 gen Inc, Redwood City, CA 94063 USA
Titolo Testata:
PLANT JOURNAL
fascicolo: 3, volume: 27, anno: 2001,
pagine: 235 - 242
SICI:
0960-7412(200108)27:3<235:AFNDMR>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA INSERTION MUTAGENESIS; ARABIDOPSIS-THALIANA; T-DNA; FUNCTIONAL GENOMICS; GENES; EXPRESSION; MUTATIONS; SEQUENCE; RNA; IDENTIFICATION;
Keywords:
reverse genetics; fast neutron mutagenesis; PCR; deletion mutants; genomics; plants;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Zhang, YL Maxygen Davis, 1105 Kennedy Pl,Suite 5, Davis, CA 95616 USA Maxygen Davis 1105 Kennedy Pl,Suite 5 Davis CA USA 95616 616 USA
Citazione:
X. Li et al., "A fast neutron deletion mutagenesis-based reverse genetics system for plants", PLANT J, 27(3), 2001, pp. 235-242

Abstract

A new reverse genetics method has been developed to identify and isolate deletion mutants for targeted plant genes. Deletion mutant libraries are generated using fast neutron bombardment. DNA samples extracted from the deletion libraries are used to screen for deletion mutants by polymerase chain reaction (PCR) using specific primers flanking the targeted genes. By adjusting PCR conditions to preferentially amplify the deletion alleles, deletionmutants were identified in pools of DNA samples, each pool containing DNA from 2592 mutant lines. Deletion mutants were obtained for 84% of targeted loci from an Arabidopsis population of 51 840 lines. Using a similar approach, a deletion mutant for a rice gene was identified. Thus we demonstrate that it is possible to apply this method to plant species other than Arabidopsis. As fast neutron mutagenesis is highly efficient, it is practical to develop deletion mutant populations with more complete coverage of the genome than obtained with methods based on insertional mutagenesis. Because fastneutron mutagenesis is applicable to all plant genetic systems, this method has the potential to enable reverse genetics for a wide range of plant species.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/10/20 alle ore 20:07:28