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Titolo:
Knock-out of the plastid ribosomal protein L11 in Arabidopsis: effects on mRNA translation and photosynthesis
Autore:
Pesaresi, P; Varotto, C; Meurer, J; Jahns, P; Salamini, F; Leister, D;
Indirizzi:
Max Planck Inst Zuchtungsforsch, Zentrum Identifikat Genfunkt Insert Mutagenese Ar, D-50829 Cologne, Germany Max Planck Inst Zuchtungsforsch Cologne Germany D-50829 Cologne, Germany Univ Munich, Inst Bot, D-80638 Munich, Germany Univ Munich Munich Germany D-80638 ch, Inst Bot, D-80638 Munich, Germany Univ Dusseldorf, Inst Biochem Pflanzen, D-40225 Dusseldorf, Germany Univ Dusseldorf Dusseldorf Germany D-40225 , D-40225 Dusseldorf, Germany Max Planck Inst Zuchtungsforsch, Abt Pflanzenzuchtung & Ertragsphysiol, D-50829 Cologne, Germany Max Planck Inst Zuchtungsforsch Cologne Germany D-50829 Cologne, Germany
Titolo Testata:
PLANT JOURNAL
fascicolo: 3, volume: 27, anno: 2001,
pagine: 179 - 189
SICI:
0960-7412(200108)27:3<179:KOTPRP>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEPENDENT GTP HYDROLYSIS; ELONGATION-FACTOR-G; ESCHERICHIA-COLI; CHLAMYDOMONAS-REINHARDTII; MESSENGER-RNA; CHLOROPLAST; SUBUNIT; GENE; THALIANA; LACKING;
Keywords:
Arabidopsis thaliana; mutant; photosynthesis; plastid ribosome; Prpl11; T-DNA; translation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Leister, D Max Planck Inst Zuchtungsforsch, Zentrum Identifikat Genfunkt Insert Mutagenese Ar, Carl Von Linne Weg 10, D-50829 Cologne, Germany Max Planck Inst Zuchtungsforsch Carl Von Linne Weg 10 Cologne Germany D-50829
Citazione:
P. Pesaresi et al., "Knock-out of the plastid ribosomal protein L11 in Arabidopsis: effects on mRNA translation and photosynthesis", PLANT J, 27(3), 2001, pp. 179-189

Abstract

The prpl11-1 mutant of Arabidopsis thaliana was identified among a collection of T-DNA tagged lines on the basis of a decrease in the effective quantum yield of photosystem II. The mutation responsible was localized to Prpl11, a single-copy nuclear gene that encodes PRPL11, a component of the largesubunit of the plastid ribosome. The amino acid sequence of Arabidopsis PRPL11 is very similar to those of L11 proteins from spinach and prokaryotes. In the prpl11-1 mutant, photosensitivity and chlorophyll fluorescence parameters are significantly altered owing to changes in the levels of thylakoid protein complexes and stromal proteins. The abundance of most plastome transcripts examined, such as those of genes coding for the photosystem II core complex and RbcL, is not decreased. Plastid ribosomal RNA accumulates inwild-type amounts, and the assembly of plastid polysomes on the transcripts of the rbcL, psbA and psbE genes remains mainly unchanged in mutant plants, indicating that lack of PRPL11 affects neither the abundance of plastid ribosomes nor their assembly into polysomes. However, in vivo translation assays demonstrate that the rate of translation of the large subunit of Rubisco (RbcL) is significantly reduced in prpl11-1 plastids. Our data suggest a major role for PRPL11 in plastid ribosome activity per se, consistent with its location near the GTPase-binding centre of the chloroplast 50S ribosomal subunit. Additional effects of the mutation, including the pale green colour of the leaves and a drastic reduction in growth rate under greenhouseconditions, are compatible with reduced levels of protein synthesis in plastids.

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Documento generato il 06/04/20 alle ore 02:11:05