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Titolo:
Detection of estrogenicity by bioassay on the mouse mammary gland in vivo
Autore:
Skarda, J;
Indirizzi:
Acad Sci Czech Republ, Inst Anim Physiol & Genet, CR-10400 Prague 10, Czech Republic Acad Sci Czech Republ Prague Czech Republic 10 Prague 10, Czech Republic
Titolo Testata:
PHYSIOLOGICAL RESEARCH
fascicolo: 3, volume: 50, anno: 2001,
pagine: 275 - 282
SICI:
0862-8408(2001)50:3<275:DOEBBO>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-HORMONE; MICE; ASSAY; RATS;
Keywords:
mammary gland; mouse; estrogenocity; bioassay; progesterone;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Skarda, J Acad Sci Czech Republ, Inst Anim Physiol & Genet, Pratelstvi 560, CR-10400Prague 10, Czech Republic Acad Sci Czech Republ Pratelstvi 560 Prague Czech Republic 10 c
Citazione:
J. Skarda, "Detection of estrogenicity by bioassay on the mouse mammary gland in vivo", PHYSL RES, 50(3), 2001, pp. 275-282

Abstract

The wide chemical diversity of estrogenic compounds precludes an accurate prediction of estrogenic activity on the basis of chemical structure or radioimmunological assay and thus requires that the potency of these compoundsis defined by bioassay. The mammary duct growth response in intact prepubertal and adult gonadectomized female and male mice of the C3H/Di strain wasused to assess the estrogenicity of synthetic compounds or their derivatives. The vehicle for tested compounds should be free of estrogenic and otherhormonal effects. Olive oil or sunflower oil exerted estrogenic activitiesand were thus unsuitable as vehicles for the tested compounds. The absenceof estrogenic activity, high solubility of different steroid hormones, andthe low incidence of the inflammatory reactions at the injection site wereachieved by using a vehicle containing benzyl alcohol, benzyl benzoate, butylhydroxyanisole, butylhydroxytoluene, ethyl oleate and ethanol. The bioassay was primarily designed to examine the effect of tested compounds on mammogenesis. The duration of hormone treatment was chosen to be long enough for induction of duct growth but too short to induce lobuloalveolar differentiation. Females were treated for 10 days, males for 15 days. The proportional volume occupied by mammary epithelial structures was estimated by the modified Chalkley's technique. The mean coefficient of variation of quantitative evaluation of 10 different mammary glands obtained by two operators varied between 3.2 and 17.4 %. The mean coefficient of variation of quintuplicate determinations of each mammary gland by one operator was 10.1 %, and 11.1 % by the other. The correlation coefficient between results of two operators was 0.994. Estrogens are primarily defined by their ability to increase the mitotic activity of female secondary sex organs. However, our results have shown that progesterone alone, if administered in a high dose, stimulates mammary growth in both intact prepubertal and OV-X female mice similarly as the synthetic progestatial steroid norethindrone with inherent estrogenic properties. In contrast, progesterone alone had no effect, in young intact or adult castrated males, but norethindrone did stimulate mammary growth. These results demonstrated that the mammary gland of males is a suitable model for estrogen screening.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 04:47:03