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Titolo:
Maintenance of differentiated function of the surfactant system in human fetal lung type II epithelial cells cultured on plastic
Autore:
Gonzales, LW; Angampalli, S; Guttentag, SH; Beers, MF; Feinstein, SI; Matlapudi, A; Ballard, PL;
Indirizzi:
Childrens Hosp, Dept Pediat, Philadelphia, PA 19104 USA Childrens Hosp Philadelphia PA USA 19104 diat, Philadelphia, PA 19104 USA Univ Penn, Dept Med, Philadelphia, PA 19104 USA Univ Penn Philadelphia PAUSA 19104 Dept Med, Philadelphia, PA 19104 USA Univ Penn, Inst Environm Med, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 ironm Med, Philadelphia, PA 19104 USA
Titolo Testata:
PEDIATRIC PATHOLOGY & MOLECULAR MEDICINE
fascicolo: 5, volume: 20, anno: 2001,
pagine: 387 - 412
SICI:
1522-7952(200109)20:5<387:MODFOT>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-SP-C; GLUCOCORTICOID REGULATION; RAT LUNG; SP-B; SP-A; TRANSCRIPTIONAL REGULATION; MESSENGER-RNA; IN-VITRO; EXPRESSION; SECRETION;
Keywords:
cAMP; glucocorticoid; human fetal lung; surfactant secretion; surfactant synthesis; type II cell culture;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Gonzales, LW Abramson Res Ctr, Div Neonatol, Rm 414,3516 Civic Ctr Blvd, Philadelphia, PA 19103 USA Abramson Res Ctr Rm 414,3516 Civic Ctr Blvd Philadelphia PA USA 19103
Citazione:
L.W. Gonzales et al., "Maintenance of differentiated function of the surfactant system in human fetal lung type II epithelial cells cultured on plastic", PEDIAT PATH, 20(5), 2001, pp. 387-412

Abstract

We report a simplified culture system for human fetal lung type II cells that maintains surfactant expression. Type II cells isolated from explant cultures of hormone-treated lungs (18-22 wk gestation) by collagenase + trypsin digestion were cultured on plastic for 4 days in serum-free medium containing dexamethasone (Dex, 10 nM) + 8-bromo-cAMP (0.1 mM) + isobutylmethylxanthine (0.1 mM) or were untreated (control). Surfactant protein (SP) mRNAs decreased markedly in control cells between days 1 and 4 of culture, but mRNA levels were high in treated cells on day 4 (SP-A, SP-B, SP-C, SP-D; 600%, 100%, 85%, 130% of day 0 content, respectively). Dex or cAMP alone increased SP-B, SP-C, and SP-D mRNAs and together had additive effects. The greatest increase in SP-A mRNA occurred with cAMP alone. Treated cells processedpro-SP-B and pro-SP-C proteins to mature forms and had a higher rate of phosphatidylcholine (PC) synthesis (2-fold) and higher saturation of PC (similar to 34% versus 27%) than controls. Only treated cells maintained secretagogue-responsive phospholipid synthesis. By electron microscopy, the treated cells retained lamellar bodies and extensive microvilli. We conclude thatDex and cAMP additively stimulate expression of surfactant components in isolated fetal type II cells, providing a simplified culture system for investigation of surfactant-related, and perhaps other, type II cell functions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/05/20 alle ore 14:57:07