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Titolo:
Genomic instability and breast cancer
Autore:
Colleu-Durel, S; Guitton, N; Nourgalieva, K; Leveque, J; Danic, B; Chenal, C;
Indirizzi:
Ctr Eugene Marquis, Unite Radiobiol, UPRES EA No 2231, F-35062 Rennes, France Ctr Eugene Marquis Rennes France F-35062 No 2231, F-35062 Rennes, France CHRU Hop Sud, F-35200 Rennes, France CHRU Hop Sud Rennes France F-35200CHRU Hop Sud, F-35200 Rennes, France Ctr Transfus Sanguine, F-35000 Rennes, France Ctr Transfus Sanguine Rennes France F-35000 uine, F-35000 Rennes, France
Titolo Testata:
ONCOLOGY REPORTS
fascicolo: 5, volume: 8, anno: 2001,
pagine: 1001 - 1005
SICI:
1021-335X(200109/10)8:5<1001:GIABC>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-DAMAGE; COMET ASSAY; IONIZING-RADIATION; GEL-ELECTROPHORESIS; INDIVIDUAL CELLS; MAMMALIAN-CELLS; REPAIR; LYMPHOCYTES; SUSCEPTIBILITY; FAMILIES;
Keywords:
genomic instability; breast cancer; comet assay;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Chenal, C Ctr Eugene Marquis, Unite Radiobiol, UPRES EA No 2231, CS 44229,F-35062 Rennes, France Ctr Eugene Marquis CS 44229 Rennes France F-35062 ennes, France
Citazione:
S. Colleu-Durel et al., "Genomic instability and breast cancer", ONCOL REP, 8(5), 2001, pp. 1001-1005

Abstract

The loss of genomic stability is accepted as being one of the most important aspects of cancer. The correlation between genomic instability and cancer proneness in cases of known genetic syndromes (e.g. ataxia telengectasia,Fanconi anemia) is well established. This study was conducted to assess genomic instability in 19 patients with sporadic breast cancer. We used the comet assay on the lymphocytes of patients before radiotherapy and/or chemotherapy. The alkaline comet assay (single-cell gel electrophoresis) is a sensitive and rapid method for detecting DNA damage (single strand breaks and alkali-labile sites) in G(o), cells, at a single-cell level. This assay wasachieved in vitro without irradiation and after exposure (dose ranging from 50 cGy to 5 Gy). The results show that the patients have higher baseline values than controls. At 2 Gy, the mean tail moment, score and the percentage of DNA in the tail increase for both groups but these values are much higher for patients. Our results show that the lymphocyte DNA of cancer patients is more damaged than that of controls with or without irradiation. Our hypothesis is that this baseline DNA damage reflect a genomic instability in sporadic breast cancer. This instability seems to increase after in vitroirradiation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 05:49:00