Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Cleavage of Fyn and Lyn in their N-terminal unique regions during induction of apoptosis: a new mechanism for Src kinase regulation
Autore:
Luciano, F; Ricci, JE; Auberger, P;
Indirizzi:
Fac Med, Equipe Labellisee Ligue Natl Canc, INSERM U526, F-06107 Nice 2, France Fac Med Nice France 2 gue Natl Canc, INSERM U526, F-06107 Nice 2, France
Titolo Testata:
ONCOGENE
fascicolo: 36, volume: 20, anno: 2001,
pagine: 4935 - 4941
SICI:
0950-9232(20010816)20:36<4935:COFALI>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-TYROSINE KINASES; FAS-MEDIATED APOPTOSIS; CELL-DEATH; HEMATOPOIETIC-CELLS; DOWN-REGULATION; DEFICIENT MICE; T-LYMPHOCYTES; B-CELLS; RECEPTOR; ACTIVATION;
Keywords:
apoptosis; Src kinases; caspases; T and B lymphocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Auberger, P Fac Med, Equipe Labellisee Ligue Natl Canc, INSERM U526, IFR50,Ave Valombrose, F-06107 Nice 2, France Fac Med IFR50,Ave Valombrose Nice France 2 107 Nice 2, France
Citazione:
F. Luciano et al., "Cleavage of Fyn and Lyn in their N-terminal unique regions during induction of apoptosis: a new mechanism for Src kinase regulation", ONCOGENE, 20(36), 2001, pp. 4935-4941

Abstract

The members of the Src kinase family are expressed in a wide variety of tissues, but some of them such as Blk, Hck, Fgr, Lck and Lyn are found primarily in hematopoietic cells. In the present study, we have undertaken experiments to test whether Src kinase cleavage and relocation is a general mechanism during induction of apoptosis. Our results indicate that Fyn and Lyn are efficiently cleaved in their unique region in hernatopoietic cells undergoing apoptosis. Fyn cleavage occurred in Fas-stimulated Jurkat T cells butFyn and Lyn were also processed in the SKW6.4 B cell line. Inhibition of caspases by Z-VAD-fmk or Ac-DEVD-CHO totally prevented Fyn and Lyn cleavage in both intact cells and in vitro. Fyn and Lyn but not Lek, Src and Hck were processed in vitro by human recombinant caspase 3 and by cellular extracts prepared from Fas-stimulated cells. Single mutation of Asp 19 or Asp 18 in the unique N-terminal domains of Fyn and Lyn respectively abolished theircleavage and relocation into the cytoplasm of apoptotic cells. When immunoprecipitated from COS cells N-terminal deleted Src kinases exhibited increased enzymatic kinase activity toward enolase. Thus, cleavage of Fyn and Lynduring induction of apoptosis represents a new mechanism for the regulation of Src kinases that may have important functional and physiological consequences.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 05:03:29