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Titolo:
Activation of p53 transcriptional activity requires ATM's kinase domain and multiple N-terminal serine residues of p53
Autore:
Turenne, GA; Paul, P; Laflair, L; Price, BD;
Indirizzi:
Harvard Univ, Sch Med, Dana Farber Canc Inst, Dept Radiat Oncol, Boston, MA 02115 USA Harvard Univ Boston MA USA 02115 Dept Radiat Oncol, Boston, MA 02115 USA
Titolo Testata:
ONCOGENE
fascicolo: 37, volume: 20, anno: 2001,
pagine: 5100 - 5110
SICI:
0950-9232(20010823)20:37<5100:AOPTAR>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
DAMAGE-INDUCED PHOSPHORYLATION; DNA-DAMAGE; ATAXIA-TELANGIECTASIA; IONIZING-RADIATION; PROTEIN-KINASE; DEPENDENT PHOSPHORYLATION; CHECKPOINT PATHWAY; CELLULAR-RESPONSE; IN-VITRO; CDS1;
Keywords:
ATM; p53; chk2; phosphorylation; bleomycin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
62
Recensione:
Indirizzi per estratti:
Indirizzo: Price, BD Harvard Univ, Sch Med, Dana Farber Canc Inst, Dept Radiat Oncol,44 BinneySt,D810A, Boston, MA 02115 USA Harvard Univ 44 Binney St,D810A Boston MA USA 02115 MA 02115 USA
Citazione:
G.A. Turenne et al., "Activation of p53 transcriptional activity requires ATM's kinase domain and multiple N-terminal serine residues of p53", ONCOGENE, 20(37), 2001, pp. 5100-5110

Abstract

The ATM protein kinase regulates the cell's response to DNA damage by regulating cell cycle checkpoints and DNA repair. ATM phosphorylates several proteins involved in the DNA-damage response, including p53. We have examinedthe mechanism by which ATM regulates p53's transcriptional activity. Here,we demonstrate that reintroduction of ATM into AT cells restores the activation of p53 by the radio-mimetic agent bleomycin. Further, p53 activation is lost when a kinase inactive ATM is used. or if the N-terminal of ATM is deleted. In addition, AT cells stably expressing ATM showed decreased sensitivity to Ionizing Radiation-induced cell killing, whereas cells expressingkinase inactive ATM or N-terminally deleted ATM were indistinguishable from AT cells. Finally, single point-mutations of serines 15, 20, 33 or 37 didnot individually block the ATM-dependent activation of p53 transcriptionalactivity by bleomycin. However, double mutations of either serines 15 and 20 or serines 33 and 37 blocked the ability of ATM to activate p53. Our results indicate that the N-terminal of ATM and ATM's kinase activity are required for activation of p53's transcriptionl activity and restoration of normal sensitivity to DNA damage. In addition, activation of p53 by ATM requires multiple serine residues in p53's transactivation domain.

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Documento generato il 20/09/20 alle ore 07:31:48