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Titolo:
L-DOPA decarboxylase association with membranes in mouse brain
Autore:
Poulikakos, P; Vassilacopoulou, D; Fragoulis, EG;
Indirizzi:
Univ Athens, Dept Biochem Mol Biol, Athens 15071, Greece Univ Athens Athens Greece 15071 t Biochem Mol Biol, Athens 15071, Greece
Titolo Testata:
NEUROCHEMICAL RESEARCH
fascicolo: 5, volume: 26, anno: 2001,
pagine: 479 - 485
SICI:
0364-3190(200105)26:5<479:LDAWMI>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID DECARBOXYLASE; INDUCED PHASE-SEPARATION; TYROSINE-HYDROXYLASE; BETA-HYDROXYLASE; MESSENGER-RNA; TRITON X-114; PROTEINS; PURIFICATION; GENE; PHENYLETHYLAMINE;
Keywords:
L-DOPA decarboxylase; mouse brain; membranes; Triton X-114; phosphatidylinositol-specific phospholipase C;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Vassilacopoulou, D Univ Athens, Dept Biochem Mol Biol, Panepistimiopolis,Kouponia, Athens 15071, Greece Univ Athens Panepistimiopolis,Kouponia Athens Greece 15071
Citazione:
P. Poulikakos et al., "L-DOPA decarboxylase association with membranes in mouse brain", NEUROCHEM R, 26(5), 2001, pp. 479-485

Abstract

This work presents evidence on the association of active DDC molecules with membranes in mammalian brain. L-DOPA decarboxylase (DDC) is generally considered to be a cytosolic enzyme. Membrane-associated DDC was detected by immunoblotting and enzymatic assay experiments. DDC activity and immunoreactivity could be partially extracted from mammalian brain membranes by detergent. Fractionation of membranes by temperature-induced phase separation in Triton X-114, resulted in the recovery of membrane-associated DDC in separation phases where integral and hydrophobic membrane proteins separate. Treatment of membranes with phosphatidylinositol-specific phospholipase C or proteinase K, did not elute membrane-associated DDC activity, suggesting thata population of DDC molecules exist embedded within membranes. The elucidation of the functional significance of the enzyme's association with membranes could provide us with new information leading to the better understanding of the biological pathways that DDC is involved in.

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Documento generato il 26/01/20 alle ore 10:03:45