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Titolo:
Permeability of proteins at the blood-brain barrier in the normal adult mouse and double transgenic mouse model of Alzheimer's disease
Autore:
Poduslo, JF; Curran, GL; Wengenack, TM; Malester, B; Duff, K;
Indirizzi:
Mayo Clin & Mayo Fdn, Mol Neurobiol Lab, Dept Neurol, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn Rochester MN USA 55905 urol, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn, Mol Neurobiol Lab, Dept Biochem Mol Biol, Rochester,MN 55905 USA Mayo Clin & Mayo Fdn Rochester MN USA 55905 Biol, Rochester,MN 55905 USA NYU, Nathan Kline Inst, Neurotransgen Lab, Orangeburg, NY 10962 USA NYU Orangeburg NY USA 10962 , Neurotransgen Lab, Orangeburg, NY 10962 USA
Titolo Testata:
NEUROBIOLOGY OF DISEASE
fascicolo: 4, volume: 8, anno: 2001,
pagine: 555 - 567
SICI:
0969-9961(200108)8:4<555:POPATB>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMYLOID PRECURSOR PROTEIN; APOLIPOPROTEIN-E; PRESENILIN-1 TRANSGENES; SENILE PLAQUES; BETA-PEPTIDE; IN-VIVO; NERVE; DEMENTIA; MICE; INCREASES;
Keywords:
blood-brain barrier; protein permeability; albumin; insulin; A beta; transgenic mice; Alzheimer's disease;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Poduslo, JF Mayo Clin & Mayo Fdn, Mol Neurobiol Lab, Dept Neurol, 200 1st St SW, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn 200 1st St SW RochesterMN USA 55905 USA
Citazione:
J.F. Poduslo et al., "Permeability of proteins at the blood-brain barrier in the normal adult mouse and double transgenic mouse model of Alzheimer's disease", NEUROBIOL D, 8(4), 2001, pp. 555-567

Abstract

The permeability of albumin, insulin, and human A beta 1-40 at the blood-brain barrier (BBB) was determined in the normal adult mouse (B6/SJL) and inthe double transgenic Alzheimer mouse (APP, PS1) by using an IN. bolus injection technique to quantify the permeability coefficient-surface area (PS)product for each protein after correction for the residual plasma volume (V-p) occupied by the protein in the blood vessels of different brain regions using a second aliquot of the same protein radiolabeled with a different isotope of iodine (I-125 vs I-131) as a vascular space marker. This technology for quantifying BBB permeability of proteins was adapted from the rat to the mouse and involved catheterizing the femoral artery and vein of the mouse instead of the brachial artery and vein as for the rat Because of the smaller blood volume in the mouse, serial sampling (20 mul) of blood from the femoral artery of the mouse was performed and directly TCA precipitated to generate a whole blood washout curve for the intact protein. When similar blood sampling techniques were used in the rat, the PS values for albuminand insulin at the BBB were similar in these two species. In the double transgenic mouse, the V-p values for albumin were significantly increased 1.4- to 1.6-fold in five of six brain regions compared to the normal adult mouse, which indicated increased adherence of albumin to vessel walls. As a result, the PS values were significantly decreased, from 1.4- to 3.2-fold, which likely reflected decreased transport of albumin by passive diffusion. In contrast, insulin, which is taken up into the brain by a receptor-mediated transport mechanism at the BBB, showed no significant difference in the V-p values but a significant increase in the PS values in four of six brain regions. This suggests a compensatory mechanism in the Alzheimer's transgenic brain whereby there is an increased permeability to insulin at the BBB. Surprisingly, there was no significant difference in the V-p or PS values for human A beta 1-40 at the BBB in the double transgenic Alzheimer mouse at24, 32, or 52 weeks of age, when there is both significant A beta levels in the plasma and amyloid burden in the brains of these animals. These data suggest that there is not an alteration in permeability to human A beta 1-40 at the BBB with increasing amyloid burden in the double transgenic Alzheimer mouse. Although these observations suggest structural alterations at the BBB, they do not support the concept of extensive BBB damage with substantial increases in BBB permeability in Alzheimer's disease. (C) 2001 Academic Press.

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Documento generato il 23/01/20 alle ore 13:09:47