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Titolo:
N-(4-hydroxyphenyl)retinamide (4-HPR) decreases neoplastic properties of human prostate cells: an agent for prevention
Autore:
Sharp, RM; Bello-DeOcampo, D; Quader, STA; Webber, MM;
Indirizzi:
Michigan State Univ, Dept Zool, E Lansing, MI 48824 USA Michigan State Univ E Lansing MI USA 48824 Zool, E Lansing, MI 48824 USA Michigan State Univ, Dept Med, E Lansing, MI 48824 USA Michigan State Univ E Lansing MI USA 48824 t Med, E Lansing, MI 48824 USA
Titolo Testata:
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
fascicolo: 1-2, volume: 496, anno: 2001,
pagine: 163 - 170
SICI:
1383-5718(20010920)496:1-2<163:N(DNPO>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
CANCER CELLS; EPITHELIAL-CELLS; CARCINOMA-CELLS; P53; LINES; GENE; CHEMOPREVENTION; DIFFERENTIATION; EXPRESSION; BREAST;
Keywords:
prostate cancer; 4-HPR; vimentin; invasion; prevention; suppressor proteins;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Webber, MM Michigan State Univ, Dept Zool, S-350 Plant Biol Bldg, E Lansing, MI 48824USA Michigan State Univ S-350 Plant Biol Bldg E Lansing MI USA 48824
Citazione:
R.M. Sharp et al., "N-(4-hydroxyphenyl)retinamide (4-HPR) decreases neoplastic properties of human prostate cells: an agent for prevention", MUT RES-GTE, 496(1-2), 2001, pp. 163-170

Abstract

The development of prostate cancer through a multistep process of carcinogenesis may have a long latent period of 20-30 years. It is possible that progression to a malignant state could be blocked or reversed during this time. This study focuses on the ability of the synthetic retinoid, N-(4-hydroxyphenyl)-retinamide (4-HPR), to reverse changes associated with malignant transformation and tumor progression, towards a normal phenotype. To examinethe responsiveness of cells at different steps of prostate carcinogenesis,three immortalized, but non-tumorigenic (RWPE-1, WPE1-7 and WPE1-10), and one human prostate carcinoma cell line (DU-145), were used. The effects of 4-HPR on cell proliferation, expression of intermediate filament proteins cytokeratin 18 and vimentin, and tumor suppressor proteins p53 and pRb were examined by immunostaining and compared. Results show that 4-HPR caused inhibition of growth in ali cell lines in a dose-dependent manner. 4-HPR induced an increase in staining for cytokeratin 18, a marker of differentiation for prostate epithelial cells. While all cell lines showed strong immunostaining for vimentin, treatment with 4-HPR for 8 days caused a marked decrease in staining for vimentin in all cell lines. In an in vitro assay, 4-HPR also caused inhibition of invasion by DU-145 cells in a dose-dependent manner. Furthermore, 4-HPR treatment was effective in significantly decreasing the abnormal nuclear staining for the tumor suppressor proteins p53 and pRb. Because 4-HPR decreased invasion-associated vimentin expression, inhibitedinvasion, and normalized p53 and pRb immunostaining, we propose that 4-HPRmay be an effective agent for secondary and tertiary prevention, i.e. promotion and progression stages, respectively, of prostate cancer. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 18:02:00