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Titolo:
Evaluation of the chemopreventive potential of retinoids using a novel in vitro human prostate carcinogenesis model
Autore:
Quader, STA; Bello-DeOcampo, D; Williams, DE; Kleinman, HK; Webber, MM;
Indirizzi:
Michigan State Univ, Dept Zool, E Lansing, MI 48824 USA Michigan State Univ E Lansing MI USA 48824 Zool, E Lansing, MI 48824 USA Michigan State Univ, Dept Med, E Lansing, MI 48824 USA Michigan State Univ E Lansing MI USA 48824 t Med, E Lansing, MI 48824 USA NIDCR, Cell Biol Sect, NIH, Bethesda, MD USA NIDCR Bethesda MD USANIDCR, Cell Biol Sect, NIH, Bethesda, MD USA
Titolo Testata:
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
fascicolo: 1-2, volume: 496, anno: 2001,
pagine: 153 - 161
SICI:
1383-5718(20010920)496:1-2<153:EOTCPO>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPITHELIAL-CELL LINES; CANCER CELLS; CARCINOMA-CELLS; N-(4-HYDROXYPHENYL)RETINAMIDE; APOPTOSIS; PC-3;
Keywords:
prostate cancer; human cell lines; chemoprevention; retinoids; invasion; progression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Webber, MM Michigan State Univ, Dept Zool, S350 Plant Biol Bldg, E Lansing, MI 48824 USA Michigan State Univ S350 Plant Biol Bldg E Lansing MI USA 48824
Citazione:
S.T.A. Quader et al., "Evaluation of the chemopreventive potential of retinoids using a novel in vitro human prostate carcinogenesis model", MUT RES-GTE, 496(1-2), 2001, pp. 153-161

Abstract

The prevalence of prostatic intraepithelial neoplasia (PIN) and latent prostatic carcinoma, representing multiple steps in carcinogenesis and progression to invasive carcinoma, makes them relevant targets for prevention. A unique family of human prostate epithelial cell lines, which mimic steps in prostate carcinogenesis and progression, were used to evaluate the chemopreventive potential of all-trans-retinoic acid (RA) and N-(4-hydroxyphenyl)retinamide (4-BPR). The effects of RA and 4-HPR on anchorage-dependent growthof an immortalized, non-tumorigenic cell line RWPE-1 and two tumorigenic cell lines, WPE1-NB14 and WPE1-NB11, derived from RWPE-1 by exposure to N-methyl-N-nitrosourea (MNU), were examined. Both tumorigenic cell lines grow more rapidly than the parent RWPE-1 cell line in monolayer culture. Further,while RWPE-1 cells do not form colonies in agar, both tumorigenic cell lines do, with a colony forming efficiency (CFE) of 1.85 and 2.04% for WPE1-NB14 and WPE1-NB11 cells, respectively. Both RA and 4-HPR inhibited anchorage-dependent growth of all cell lines and anchorage-independent growth of WPE1-NB14 and WPE1-NB11 cells, in a dose-dependent manner, however, 10 times more RA than 4-HPR was required to produce the same effect. RWPE-1 cells arenot invasive but WPE1-NB11 cells are significantly more invasive than WPE1-NB14 cells. Both RA and 4-HPR inhibited invasion in vitro by WPE1-NB11 andWPE1-NB14 cells where the more malignant WPE1-NB11 cells showed greater inhibition of invasion by 4-HPR than by RA. Overall, 4-HPR was more effectivethan RA in inhibiting growth and invasion but the response varied amongst the cell lines. These three cell lines mimic progressive steps in carcinogenesis and progression, from immortalized, non-tumorigenic RWPE-1 cells, to the less malignant WPE1-NB14 to the more malignant WPE1-NB11 cells, and provide powerful models for studies on secondary and tertiary prevention, i.e.promotion and progression stages, respectively, of prostate cancer. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 19:12:07