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Titolo:
High mutation rate and mutational bias at (TAA)(n) microsatellite loci in chickpea (Cicer arietinum L.)
Autore:
Udupa, SM; Baum, M;
Indirizzi:
ICARDA, Aleppo, Syria ICARDA Aleppo SyriaICARDA, Aleppo, Syria
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 6, volume: 265, anno: 2001,
pagine: 1097 - 1103
SICI:
1617-4615(200108)265:6<1097:HMRAMB>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
DROSOPHILA-MELANOGASTER; GENETIC DIVERSITY; MAJOR MECHANISM; EVOLUTION; REPEATS; GENOME; DIRECTIONALITY; POPULATIONS; INSTABILITY; CONSTRAINTS;
Keywords:
mutation rate; mutational bias; microsatellites; ageing; plants;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Baum, M ICARDA, POB 5466, Aleppo, Syria ICARDA POB 5466 Aleppo SyriaICARDA, POB 5466, Aleppo, Syria
Citazione:
S.M. Udupa e M. Baum, "High mutation rate and mutational bias at (TAA)(n) microsatellite loci in chickpea (Cicer arietinum L.)", MOL GENET G, 265(6), 2001, pp. 1097-1103

Abstract

Microsatellites, very short tandemly repeated DNA sequences, are being extensively used in evolutionary genetics and molecular breeding of crop plants, because of their high degree of allelic variability, which is presumablycaused by a high rate of mutation that changes microsatellite array length. In humans and various animals, mutation rates vary greatly and fall within the range of 10(-3) to 10(-6). In plants, the mutation rate at microsatellite loci seems to be higher than in animals, but no experimental estimatesare available yet. Here, we report high spontaneous mutation rates (mu) and mutational bias at fifteen perfect (TAA)(n) microsatellite loci in inbredpopulations of chickpea. We show a significantly higher mutation rate, averaged across all loci, in the long-lived variety Ghab 2 (mu = 1.0 x 10(-2) ; detected in 16,050 allele-generations) compared to the variety Syrian Local (mu = 3.9 x 10(-3); detected in 15,600 allele-generations), which has a short life-span, with the majority of mutants (96.9%) in both varieties differing by 1 repeat unit. Compared to animals, higher mutation rates in chickpea are likely to be due to the presence of long (TAA)n microsatellite repeat arrays and the larger number of DNA replications that meristematic initials of the plants undergo before reaching the reproductive phase. Thus, the long-lived variety undergoes more DNA replications, resulting in an accumulation of more mutations than in the variety with the shorter life-span.

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Documento generato il 06/07/20 alle ore 05:56:30