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Titolo:
Proteins that genetically interact with the Saccharomyces cerevisiae transcription factor Gal11p emphasize its role in the initiation-elongation transition
Autore:
Badi, L; Barberis, A;
Indirizzi:
Univ Zurich, Inst Mol Biol, CH-8057 Zurich, Switzerland Univ Zurich Zurich Switzerland CH-8057 Biol, CH-8057 Zurich, Switzerland
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 6, volume: 265, anno: 2001,
pagine: 1076 - 1086
SICI:
1617-4615(200108)265:6<1076:PTGIWT>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA-POLYMERASE-II; C-TERMINAL DOMAIN; NUCLEOTIDE-EXCISION-REPAIR; IN-VITRO; YEAST GAL11; P-TEFB; KINASE; GENE; HOLOENZYME; ACTIVATION;
Keywords:
multicopy suppressors; GAL11; RNA polymerase II holoenzyme; transcription initiation; transcription elongation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Barberis, A Univ Zurich, Inst Mol Biol, CH-8057 Zurich, Switzerland Univ Zurich Zurich Switzerland CH-8057 7 Zurich, Switzerland
Citazione:
L. Badi e A. Barberis, "Proteins that genetically interact with the Saccharomyces cerevisiae transcription factor Gal11p emphasize its role in the initiation-elongation transition", MOL GENET G, 265(6), 2001, pp. 1076-1086

Abstract

The GAL11 gene encodes a transcription factor that is a component of the SRB/Mediator subcomplex of the RNA polymerase II holoenzyme in the yeast Saccharomyces cerevisiae.. In agreement with this biochemical characterization, Gal11p has been found to be required for optimal production of mRNA from many yeast promoters, and recessive mutations in GAL11 have been shown to cause pleiotropic defects. Despite this progress, the role of Gal11p in generegulation remains largely unknown. In a multicopy suppressor analysis of a gal11 Delta mutation we have identified genes encoding proteins that are part of, or can interact with, the RNA polymerase II transcription complex,as well as factors involved in cell cycle regulation. Among the suppressors that are clearly related to the transcriptional apparatus, Gal11p genetically interacts with components of the SRB/Mediator complex, as well as withfactors such as TFIIE and TFIIH that are required for promoter clearance and transcription elongation by RNA polymerase II. These findings, taken together with published results of biochemical and genetic analyses, suggest arole for Gal11p at the interface between the SRB/Mediator complex and the general transcription factors TFIIE and TFIIH, which modulate, via phosphorylation of the CTD, the activity of the RNA polymerase.

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Documento generato il 09/07/20 alle ore 01:34:04