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Titolo:
sna41(goal), a novel mutation causing G1/S arrest in fission yeast, is defective in a CDC45 homolog and interacts genetically with pol alpha
Autore:
Uchiyama, M; Arai, K; Masai, H;
Indirizzi:
Univ Tokyo, Inst Med Sci, Dept Mol & Dev Biol, Minato Ku, Tokyo 1088639, Japan Univ Tokyo Tokyo Japan 1088639 Dev Biol, Minato Ku, Tokyo 1088639, Japan Japan Sci & Technol Corp JST, CREST Program, Tokyo, Japan Japan Sci & Technol Corp JST Tokyo Japan T, CREST Program, Tokyo, Japan Tokyo Metropolitan Inst Med Sci, Dept Cell Biol, Bunkyo Ku, Tokyo 1138613,Japan Tokyo Metropolitan Inst Med Sci Tokyo Japan 1138613 Tokyo 1138613,Japan
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 6, volume: 265, anno: 2001,
pagine: 1039 - 1049
SICI:
1617-4615(200108)265:6<1039:SANMCG>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA-REPLICATION ORIGINS; S-PHASE; SCHIZOSACCHAROMYCES-POMBE; CELL-CYCLE; BUDDING YEAST; MCM PROTEIN; MINICHROMOSOME MAINTENANCE; CHROMOSOMAL REPLICATION; POLYMERASE-DELTA; GENE-PRODUCT;
Keywords:
fission-yeast; cell cycle; initiation of DNA replication; prereplicative complex; DNA polymerase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Masai, H Univ Tokyo, Inst Med Sci, Dept Mol & Dev Biol, Minato Ku, 4-6-1 Shirokanedai, Tokyo 1088639, Japan Univ Tokyo 4-6-1 Shirokanedai Tokyo Japan 1088639 1088639, Japan
Citazione:
M. Uchiyama et al., "sna41(goal), a novel mutation causing G1/S arrest in fission yeast, is defective in a CDC45 homolog and interacts genetically with pol alpha", MOL GENET G, 265(6), 2001, pp. 1039-1049

Abstract

Proteins involved in the initiation of DNA replication play critical rolesin the assembly and loading of replication complexes at replication origins. To gain further insight into the regulation of initiation, we screened in fission yeast for temperature-sensitive mutants which arrested at the G1/S boundary, and isolated nine mutants which arrested with a 1C DNA content at 36 degreesC. By linkage analysis, two complementation groups were identified which were not allelic to known G1 arrest mutations. One of the mutants isolated, sna41(goal), arrested with a G1 DNA content and expressed a pleiomorphic phenotype, i.e., a mixture of cut and cdc phenotypes, at 36 degreesC. The point of arrest was identified as after START but before the hydroxyurea-induced block, by taking advantage of the mutant rad26.a14, which has a defect in an early S phase-specific checkpoint, and by performing reciprocal shift experiments. sna41(goal) is allelic to sna41(+), which is homologous to the CDC45 gene of budding yeast, and the mutation lies in a motif that is highly conserved in Cdc45-related proteins. The temperature sensitivity of the sna41(goal) mutant can be suppressed to some extent by ts mutations in polo. Our genetic results are consistent with a model in which Cdc45 plays crucial roles in the assembly of the replication apparatus at replication origins.

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Documento generato il 10/07/20 alle ore 00:00:09