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Titolo:
Multiple allelism as a control mechanism in metabolic pathways: GBSSI allelic composition affects the activity of granule-bound starch synthase I andstarch composition in potato
Autore:
van de Wal, MHBJ; Jacobsen, E; Visser, RGF;
Indirizzi:
Univ Wageningen & Res Ctr, Grad Sch Expt Plant sci, Lab Plant Breeding, NL-6700 AJ Wageningen, Netherlands Univ Wageningen & Res Ctr Wageningen Netherlands NL-6700 AJ Netherlands
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 6, volume: 265, anno: 2001,
pagine: 1011 - 1021
SICI:
1617-4615(200108)265:6<1011:MAAACM>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOLANUM-TUBEROSUM-L; 3 WX GENES; AMYLOSE CONTENT; AMYLOPECTIN; EXPRESSION; WHEAT; ENDOSPERM; MUTANT; LOCUS;
Keywords:
GBSSI; multiple allelism; metabolic pathway; starch biosynthesis; amylose;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Visser, RGF Univ Wageningen & Res Ctr, Grad Sch Expt Plant sci, Lab Plant Breeding, POB 386, NL-6700 AJ Wageningen, Netherlands Univ Wageningen & ResCtr POB 386 Wageningen Netherlands NL-6700 AJ
Citazione:
M.H.B.J. van de Wal et al., "Multiple allelism as a control mechanism in metabolic pathways: GBSSI allelic composition affects the activity of granule-bound starch synthase I andstarch composition in potato", MOL GENET G, 265(6), 2001, pp. 1011-1021

Abstract

Multiple allelism in heterozygous autopolyploid species like potato not only occurs for genes that affect morphological characteristics but also for genes involved in metabolic pathways. Based on a combination of Southern and PCR analyses, at least eight alleles encoding granule-bound starch synthase I (GBSSI), which is responsible for amylose biosynthesis, have been identified in potato. These alleles were grouped into four classes, distinguishable by Southern analysis, and subdivided based on PCR. Despite the heterozygous and polyploid character of potato it was possible to assign variationin GBSSI activity to the allelic composition at the GBSSI loci within a large population of Solanum tuberosum cultivars and Solanum breeding lines. Moreover, the availability of an amf allele made it possible to reduce heterogeneity and enabled us to demonstrate an effect of GBSSI allelic composition on amylose content. The major difference between the alleles identified was the absence or presence of a 140-bp fragment at a site 0.5 kb upstream of the ATG start codon of the gene for GBSSI. The absence of this 140-bp fragment had a major effect on GBSSI activity and amylose content, while the presence of small deletions and simple sequence repeats had no obvious effect.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 14:50:28