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Titolo:
A homologue of the Rad18 postreplication repair gene is required for DNA damage responses throughout the fission yeast cell cycle
Autore:
Verkade, HM; Teli, T; Laursen, LV; Murray, JM; OConnell, MJ;
Indirizzi:
Peter MacCallum Canc Inst, Trescowthick Res Labs, Melbourne, Vic 8006, Australia Peter MacCallum Canc Inst Melbourne Vic Australia 8006 ic 8006, Australia Univ Sussex, Sch Biol Sci, Brighton BN1 9QG, E Sussex, England Univ Sussex Brighton E Sussex England BN1 9QG BN1 9QG, E Sussex, England Univ Melbourne, Dept Genet, Parkville, Vic 3052, Australia Univ MelbourneParkville Vic Australia 3052 arkville, Vic 3052, Australia
Titolo Testata:
MOLECULAR GENETICS AND GENOMICS
fascicolo: 6, volume: 265, anno: 2001,
pagine: 993 - 1003
SICI:
1617-4615(200108)265:6<993:AHOTRP>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
UBIQUITIN-CONJUGATING ENZYME; UV-IRRADIATED YEAST; SACCHAROMYCES-CEREVISIAE; SCHIZOSACCHAROMYCES-POMBE; ESCHERICHIA-COLI; PROTEIN-DEGRADATION; RECA PROTEIN; END RULE; MECHANISM; CHECKPOINT;
Keywords:
DNA repair; fission yeast; DNA damage; rhp18; S. pombe;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: O'Connell, MJ Peter MacCallum Canc Inst, Trescowthick Res Labs, Locked Bag1,ABeckett St, Melbourne, Vic 8006, Australia Peter MacCallum Canc Inst Locked Bag 1,ABeckett St Melbourne Vic Australia 8006
Citazione:
H.M. Verkade et al., "A homologue of the Rad18 postreplication repair gene is required for DNA damage responses throughout the fission yeast cell cycle", MOL GENET G, 265(6), 2001, pp. 993-1003

Abstract

Cells activate DNA repair pathways and cell cycle checkpoints when they suffer damage to their genome. They also activate tolerance pathways that facilitate survival. In Escherichia coli, a mechanism known as postreplicationrepair (PRR) is used to bypass lesions that would otherwise present a physical block to DNA polymerase. PRR has also been proposed to occur in eukaryotic cells, although the partitioning of DNA synthesis to a discrete S-phase would suggest that it is only operative within a defined period of the cell cycle. Eukaryotic PRR has been most extensively studied in the budding yeast Saccharomyces cerevisiae. Two important genes for components of this repair pathway are RAD6, which encodes an ubiquitin-conjugating enzyme, and RAD18, which encodes a RING-finger protein and forms a heterodimer with Rad6p. Rad18p can also bind to DNA. We report here the identification of the Schizosaccharomyces pombe homologue of RAD18, which we have denoted rhp18. rhp18 mutants are hypersensitive to DNA-damaging agents, but show this hypersensitivity throughout the cell cycle. rhp18 mutants are characterised by alonger than usual DNA damage checkpoint arrest that is required for their residual viability following irradiation. Genetic analyses show that rhp18 controls a unique DNA damage repair/tolerance pathway that extends beyond the requirement to tolerate damage during S-phase, suggesting a broader definition of the function of this eukaryotic PRR protein.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 14:03:48